Literature summary for 2.7.3.3 extracted from

Ooi, C.P.; Rotureau, B.; Gribaldo, S.; Georgikou, C.; Julkowska, D.; Blisnick, T.; Perrot, S.; Subota, I.; Bastin, P.
The flagellar arginine kinase in Trypanosoma brucei is important for infection in tsetse flies (2015), PLoS ONE, 10, e0133676.

Search for more literature for 2.7.3.3

Cloned(Commentary)

Cloned (Comment)	Organism
gene AK1 or Tb927.9.6170, located on chromosome 9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression of full-length and truncated versions of epitope tagged isozyme AK1	Trypanosoma brucei

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a stable isozyme AK1 knockout DELTAak1 line	Trypanosoma brucei

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
flagellum	isozyme AK1, the 22 N-terminal amino acids are responsible for flagellum targeting. The flagellar targeting sequence of AK1 shares close similarity with that of calflagins. The flagellar localisation of AK1 persists throughout the Trypanosoma brucei life cycle, overview	Trypanosoma brucei	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	x * 45000, SDS-PAGE	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	Q9U420	gene AK1 = gene Tb927.9.6170	-

Subunits

Subunits	Comment	Organism
?	x * 45000, SDS-PAGE	Trypanosoma brucei

Synonyms

Synonyms	Comment	Organism
AK1	-	Trypanosoma brucei
Tb927.9.6170	-	Trypanosoma brucei

pI Value

Organism	Comment	pI Value Maximum	pI Value
Trypanosoma brucei	sequence calculation	-	4.9

General Information

General Information	Comment	Organism
evolution	arginine kinase genes in trypanosomatids, phylogenetic analysis, overview	Trypanosoma brucei
malfunction	RNAi knockdown of all three arginine kinase isozymes induces a growth defect that is more prominent when the cells are exposed to oxidative stress. Loss of flagellar isozyme AK1 reduces swim velocity without visible alteration of flagellar morphology. The absence of isozyme AK1 results in reduced infectivity by procyclic trypanosomes for tsetse flies	Trypanosoma brucei
additional information	isozyme AK1 has two insertions of respectively 22 and 26 amino acids at the N- and C-terminus	Trypanosoma brucei
physiological function	isozyme AK1 confers a competitive advantage in infections of tsetse flies in midgut by the parasite, overview	Trypanosoma brucei

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Release 2023.1 (January 2023)