Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Macrobrachium rosenbergii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | the enzyme activity is inhibited at 10-200 mM | Macrobrachium rosenbergii | |
ATP | the enzyme activity is inhibited at 100 mM | Macrobrachium rosenbergii | |
L-Glucose | AK-1 activity does not show significant variation after supplementation with 10 mM L-glucose. However, AK-1 activity decreases significantly when L-glucose concentration is higher than 50 mM and almost all MrAK-1 activity is lost after treatment with 200 mM L-glucose | Macrobrachium rosenbergii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, calculated from amino acid sequence | Macrobrachium rosenbergii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macrobrachium rosenbergii | E2JE77 | - |
- |
Purification (Comment) | Organism |
---|---|
the crude AK-1 fusion protein fused with maltose binding protein is purified using pMAL protein fusion and purification system protocol | Macrobrachium rosenbergii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Macrobrachium rosenbergii | - |
eyestalk | lowest expression | Macrobrachium rosenbergii | - |
gill | - |
Macrobrachium rosenbergii | - |
hemocyte | - |
Macrobrachium rosenbergii | - |
hepatopancreas | - |
Macrobrachium rosenbergii | - |
intestine | - |
Macrobrachium rosenbergii | - |
leg | walking leg | Macrobrachium rosenbergii | - |
muscle | highest expression | Macrobrachium rosenbergii | - |
pleopod | - |
Macrobrachium rosenbergii | - |
stomach | - |
Macrobrachium rosenbergii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Macrobrachium rosenbergii | ADP + Nomega-phospho-L-Arg | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, calculated from amino acid sequence | Macrobrachium rosenbergii |
Synonyms | Comment | Organism |
---|---|---|
AK-1 | - |
Macrobrachium rosenbergii |
arginine kinase-1 | - |
Macrobrachium rosenbergii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Macrobrachium rosenbergii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | - |
Macrobrachium rosenbergii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Macrobrachium rosenbergii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | - |
Macrobrachium rosenbergii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Macrobrachium rosenbergii | calculated from amino acid sequence | - |
6.1 |
Organism | Comment | Expression |
---|---|---|
Macrobrachium rosenbergii | the levels of AK-1 mRNA transcripts sharply increase until 12 h post-injection with IHHNV | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays an important role in the coupling of energy production and utilization and the immune response in shrimps | Macrobrachium rosenbergii |