Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | after treatment with 10 mM ATP, the enzyme activity significantly increases | Penaeus vannamei |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) codon plus cells by prokaryotic expression plasmid pGEX-4T-2 as glutathione S-transferase arginine kinase fusion protein | Penaeus vannamei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | the enzyme activity is inhibited by 10 mM 2-oxoglutarate | Penaeus vannamei | |
ATP | the enzyme is inhibited by 200 mM ATP | Penaeus vannamei | |
D-glucose | the enzyme is inhibited by 50 mM D-glucose, almost all arginine kinase activity is lost after treatment with 200 mM D-glucose | Penaeus vannamei | |
additional information | arginine kinase activity does not show significant variation after incubated with 10-200 mM L-citrulline, L-ornaline, and glycerol | Penaeus vannamei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Penaeus vannamei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Penaeus vannamei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penaeus vannamei | B0FRF9 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose 4B column chromatography | Penaeus vannamei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eyestalk | very low mRNA expression | Penaeus vannamei | - |
gill | very low mRNA expression | Penaeus vannamei | - |
heart | - |
Penaeus vannamei | - |
hemocyte | - |
Penaeus vannamei | - |
hepatopancreas | very low mRNA expression | Penaeus vannamei | - |
intestine | very low mRNA expression | Penaeus vannamei | - |
muscle | highest mRNA expression in the muscle | Penaeus vannamei | - |
nerve | - |
Penaeus vannamei | - |
skin | subcuticular skin, lowest mRNA expression in the skin | Penaeus vannamei | - |
stomach | - |
Penaeus vannamei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Penaeus vannamei | ADP + Nomega-phospho-L-Arg | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ATP: arginine N-phosphotransferase | - |
Penaeus vannamei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Penaeus vannamei |
General Information | Comment | Organism |
---|---|---|
physiological function | arginine kinase may play an important role in the coupling of energy production and utilization and the immune response in shrimps | Penaeus vannamei |