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Literature summary for 2.7.3.3 extracted from

  • Wu, Q.Y.; Li, F.; Wang, X.Y.
    Evidence that amino-acid residues are responsible for substrate synergism of locust arginine kinase (2008), Insect Biochem. Mol. Biol., 38, 59-65.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes (Y75F, Y75D, P272G, P272R, Y75F/P272G and Y75D/P272R) in Escherichia coli BL21 (DE3) Locusta migratoria manilensis

Protein Variants

Protein Variants Comment Organism
P272G the synergism is almost completely lost. Fluorescence spectrum shows a red shift Locusta migratoria manilensis
P272R the synergism is almost completely lost. Fluorescence spectrum shows a red shift Locusta migratoria manilensis
Y75D mutant shows strong synergism. Fluorescence spectrum shows a red shift Locusta migratoria manilensis
Y75D/P272R characteristics similar to those of the wild-type enzyme Locusta migratoria manilensis
Y75F mutant shows strong synergism. Fluorescence spectrum shows a red shift Locusta migratoria manilensis
Y75F/P272G the synergism is almost completely lost. Fluorescence spectrum shows a red shift Locusta migratoria manilensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.91
-
L-Arg pH 8.6, 30°C, mutant enzyme Y75D Locusta migratoria manilensis
0.94
-
L-Arg pH 8.6, 30°C, mutant enzyme Y75F Locusta migratoria manilensis
0.94
-
L-Arg pH 8.6, 30°C, native arginine kinase Locusta migratoria manilensis
0.95
-
L-Arg pH 8.6, 30°C, recombinant arginine kinase Locusta migratoria manilensis
1.099
-
ATP pH 8.6, 30°C, mutant enzyme Y75F Locusta migratoria manilensis
1.27
-
ATP pH 8.6, 30°C, recombinant arginine kinase Locusta migratoria manilensis
1.29
-
ATP pH 8.6, 30°C, native arginine kinase Locusta migratoria manilensis
1.36
-
ATP pH 8.6, 30°C, mutant enzyme Y75D Locusta migratoria manilensis
1.53
-
L-Arg pH 8.6, 30°C, mutant enzyme Y75D/P272R Locusta migratoria manilensis
1.65
-
ATP pH 8.6, 30°C, mutant enzyme P272G Locusta migratoria manilensis
1.93
-
ATP pH 8.6, 30°C, mutant enzyme Y75D/P272R Locusta migratoria manilensis
2.84
-
ATP pH 8.6, 30°C, mutant enzyme P272R Locusta migratoria manilensis
2.85
-
L-Arg pH 8.6, 30°C, mutant enzyme P272R Locusta migratoria manilensis
3.78
-
L-Arg pH 8.6, 30°C, mutant enzyme P272G Locusta migratoria manilensis
3.98
-
L-Arg pH 8.6, 30°C, mutant enzyme Y75F/P272G Locusta migratoria manilensis
4.2
-
ATP pH 8.6, 30°C, mutant enzyme Y75F/P272G Locusta migratoria manilensis

Organism

Organism UniProt Comment Textmining
Locusta migratoria manilensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg synergism in substrate binding Locusta migratoria manilensis ADP + omega-N-phospho-L-Arg
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60.3
-
ATP pH 8.6, 30°C, mutant enzyme P272R Locusta migratoria manilensis
74.6
-
ATP pH 8.6, 30°C, mutant enzyme Y75F/P272G Locusta migratoria manilensis
79.2
-
ATP pH 8.6, 30°C, mutant enzyme P272G Locusta migratoria manilensis
126.2
-
ATP pH 8.6, 30°C, mutant enzyme Y75F Locusta migratoria manilensis
140.7
-
ATP pH 8.6, 30°C, mutant enzyme Y75D Locusta migratoria manilensis
141.3
-
ATP pH 8.6, 30°C, mutant enzyme Y75D/P272R Locusta migratoria manilensis
159.4
-
ATP pH 8.6, 30°C, recombinant arginine kinase Locusta migratoria manilensis
163
-
ATP pH 8.6, 30°C, native arginine kinase Locusta migratoria manilensis