Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a fusion with maltose-binding protein | Cissites cephalotes |
expression of Cissites arginine kinase protein in Escherichia coli as a fusion with maltose-binding protein | Cissites cephalotes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.95 | - |
ATP | pH 8, 25°C | Cissites cephalotes | |
0.95 | - |
ATP | in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C | Cissites cephalotes | |
1.01 | - |
L-Arg | pH 8, 25°C | Cissites cephalotes | |
1.01 | - |
L-Arg | in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C | Cissites cephalotes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Cissites cephalotes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cissites cephalotes | - |
- |
- |
Cissites cephalotes | A4F2K7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + omega-N-phospho-L-Arg | function: five residues predicted to interact with the substrate arginine (S77, Y82, E239, C285 and E328), and five residues predicted to interact with the substrate ADP (R138, R140, R243, R294 and R323). Arginine (or phosphagen) and MgATP (or MgADP), typically exhibit synergistic binding to arginine kinase | Cissites cephalotes | ATP + L-arginine | - |
r | |
ATP + L-Arg | - |
Cissites cephalotes | ADP + Nomega-phospho-L-Arg | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.02 | - |
ATP | pH 8, 25°C | Cissites cephalotes | |
2.02 | - |
L-Arg | pH 8, 25°C | Cissites cephalotes | |
2.02 | - |
L-Arg | in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C | Cissites cephalotes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Cissites cephalotes |