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Literature summary for 2.7.3.3 extracted from

  • Storey, K.B.
    Purification and characterization of arginine kinase from the mantle muscle of the squid, Symplectoteuthis oualaniensis. Role of the phosphagen/phosphagen kinase system in a highly aerobic muscle (1977), Arch. Biochem. Biophys., 179, 518-526.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Sthenoteuthis oualaniensis
Cl-
-
Sthenoteuthis oualaniensis
Cu2+
-
Sthenoteuthis oualaniensis
Fe2+
-
Sthenoteuthis oualaniensis
K+ 200 mM, 50% inhibition Sthenoteuthis oualaniensis
Na+ 200 mM, 50% inhibition Sthenoteuthis oualaniensis
NADH noncompetitive Sthenoteuthis oualaniensis
NH4+ 200 mM, 50% inhibition Sthenoteuthis oualaniensis
SO42-
-
Sthenoteuthis oualaniensis
Zn2+
-
Sthenoteuthis oualaniensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
ADP pH 7.2, 25°C Sthenoteuthis oualaniensis
0.8
-
ATP pH 7.6, 25°C Sthenoteuthis oualaniensis
1
-
L-Arg pH 7.6, 25°C Sthenoteuthis oualaniensis
3.5
-
Nomega-phospho-L-Arg pH 7.2, 25°C Sthenoteuthis oualaniensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 4% of the activation with Mg2+ Sthenoteuthis oualaniensis
Mg2+ Km: 0.6 mM Sthenoteuthis oualaniensis
Mg2+ divalent cation requirement is satisfied by Mg2+ or Mn2+. Km-value for Mg2+: 0.6 mM Sthenoteuthis oualaniensis
Mg2+ highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate Sthenoteuthis oualaniensis
Mn2+ Km: 0.08 mM Sthenoteuthis oualaniensis
Mn2+ divalent cation requirement is satisfied by Mg2+ or Mn2+. Km-value for Mn2+: 0.08 mM Sthenoteuthis oualaniensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
gel filtration Sthenoteuthis oualaniensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-Arg Sthenoteuthis oualaniensis
-
ADP + Nomega-phospho-L-Arg
-
?

Organism

Organism UniProt Comment Textmining
Sthenoteuthis oualaniensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sthenoteuthis oualaniensis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
Sthenoteuthis oualaniensis ADP + Nomega-phospho-L-Arg
-
?
ATP + L-Arg
-
Sthenoteuthis oualaniensis ADP + Nomega-phospho-L-Arg
-
r
GDP + Nomega-phospho-L-Arg 10% of the activity with ADP Sthenoteuthis oualaniensis GTP + L-Arg
-
?
UDP + Nomega-phospho-L-Arg 10% of the activity with ADP Sthenoteuthis oualaniensis UTP + L-Arg
-
?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Sthenoteuthis oualaniensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
synthesis of ATP, 20 mM phosphate buffer Sthenoteuthis oualaniensis
6.9
-
synthesis of ATP, 20 mM Tris-HCl buffer Sthenoteuthis oualaniensis
8.2
-
synthesis of N-phospho-L-arginine, 20 mM Tris-HCl buffer or 20 mM phosphate buffer Sthenoteuthis oualaniensis

pH Range

pH Minimum pH Maximum Comment Organism
5.9 6.8 pH 5.9: about 35% of maximal activity, pH 6.8: about 55% of maximal activity, synthesis of ATP, 20 mM phosphate buffer Sthenoteuthis oualaniensis
6.5 7.3 pH 6.5: about 45% of maximal activity, pH 9.0: about 45% of maximal activity, synthesis of ATP, 20 mM Tris-HCl buffer Sthenoteuthis oualaniensis
7.5 9 about 50% of maximal activity at pH 7.5 and pH 9.0, synthesis of N-phospho-L-arginine, 20 mM Tris-HCl buffer Sthenoteuthis oualaniensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
Zn2+
-
Sthenoteuthis oualaniensis
0.15
-
NADH
-
Sthenoteuthis oualaniensis
0.5
-
Cu2+
-
Sthenoteuthis oualaniensis
0.85
-
Ca2+
-
Sthenoteuthis oualaniensis
1.5
-
Fe2+
-
Sthenoteuthis oualaniensis
100
-
SO42-
-
Sthenoteuthis oualaniensis