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Literature summary for 2.7.3.2 extracted from
- Purification and stability of octameric mitochondrial creatine kinase isoform from herring (Clupea harengus) organ of vision (2015), Comp. Biochem. Physiol. B, 185, 16-23.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | transition state analogue complex substrates inhibit the dimeric but not the octameric enzyme; transition state analogue complex substrates inhibit the dimeric but not the octameric enzyme | Clupea harengus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | there are two mitochondrial isoforms of CK: s-type (sarcomeric, expressed in skeletal and heart muscles only) and u-type (ubiquitous, expressed in many other tissues), which both exist as octamers in fish as well as in higher vertebrates and invertebrates. The mitochondrial s-type, rather than u-type, is predominantly expressed in herring eye | Clupea harengus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Clupea harengus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
dimeric enzyme form, gel filtration | Clupea harengus |
| 335000 | - |
octameric enzyme form of isozyme MtCK, gel filtration | Clupea harengus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | Clupea harengus | - |
ADP + phosphocreatine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clupea harengus | - |
isozyme uMtCK | - |
| Clupea harengus | A0A0A1IVI8 | muscle-type CK; isozyme sMtCK, muscle-type CK, gene ckm | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isolation of the octameric enzyme form from eyes by gel filtration, and affinity and anion exchange chromatgraphy | Clupea harengus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | four CK isoforms: one mitochondrial MtCK and three cytosolic isoforms: muscle-type CK (cyt-MM-CK), brain-type CK (cyt-BB-CK) and muscle-brain-type CK (cyt-MB-CK). The mitochondrial s-type, rather than u-type, is predominantly expressed in herring eye | Clupea harengus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 181 | - |
purified native octameric enzyme, pH and temperature not specified in the publication | Clupea harengus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | - |
Clupea harengus | ADP + phosphocreatine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | cytosolic isozyme | Clupea harengus |
| More | MtCK generally exists as an octamer, but a dynamic octamer/dimer equilibrium exists in vitro depending on various parameters like MtCK concentration, temperatures and pH. Enzyme activity and temperature stability /sensitivity are higher for the octameric enzyme than for the dimeric enzyme. MtCK dimers are very sensitive and inactivate after exposure to temperatures above 30°C | Clupea harengus |
| octamer | mitochondrial isozyme MtCK | Clupea harengus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| s-type CK | - |
Clupea harengus |
| sarcomeric CK | - |
Clupea harengus |
| sMtCK | - |
Clupea harengus |
| u-type CK | - |
Clupea harengus |
| ubiquitous CK | - |
Clupea harengus |
| uMtCK | - |
Clupea harengus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | - |
Clupea harengus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
60 | activity range, profile overview | Clupea harengus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme activity and temperature stability /sensitivity are higher for the octameric enzyme than for the dimeric enzyme. MtCK dimers are very sensitive and inactivate after exposure to temperatures above 30°C | Clupea harengus |
- |
60 | the enzyme acctivity is stable from 0°C up to 45°C, inactivation at 65°C | Clupea harengus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Clupea harengus | |
| ATP | - |
Clupea harengus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the MtCK gene is likely basal and ancestral and has evolved very early in metazoan evolution | Clupea harengus |
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