Literature summary for 2.7.3.2 extracted from

Wu, C.L.; Li, Y.H.; Lin, H.C.; Yeh, Y.H.; Yan, H.Y.; Hsiao, C.D.; Hui, C.F.; Wu, J.L.
Activity and function of rabbit muscle-specific creatine kinase at low temperature by mutation at gly268 to asn268 (2011), Comp. Biochem. Physiol. B, 158, 189-198.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of the mutant G268N are successfully raised to study the fine structural change around the active site. Results show that one residue mutation in can cause the mutant G268N to exhibit cold-adapted enzyme properties comparable to those of Cyprinus carpio M1-creatine kinase	Oryctolagus cuniculus

Protein Variants

Protein Variants	Comment	Organism
A267H	at pH 7.1 mutant shows 30% higher specific activity than the wild-type at 35°C, in contrast to mutant G268N this mutant shows no cold-adapted characteristics	Oryctolagus cuniculus
G286N	Km values of the rabbit creatine kinase G268N mutant are similar to those of the wild-type rabbit enzyme, circular dichroism spectra show that the overall secondary structures of the mutant enzyme, at pH 8.0 and 5 °C, are almost identical to the carp M1-creatine kinase enzyme. At pH 7.4-8.0 and 35-10 °C, with a smaller substrate, dADP, specific activities of the mutant enzyme are consistently higher than the wild-type rabbit enzyme. At pH 7.1 mutant shows 23% higher specific activity than the wild-type at 35°C. At pH 7.7 and pH 8.0 at 10°C mutant G268N exhibits 2 to 2.5fold higher specific activity than the wild-type, comparable to Cyprinus carpio M1-creatine kinase. Km and kcat values similar to wild-type	Oryctolagus cuniculus
P270G	at pH 7.1 mutant shows 30% higher specific activity than the wild-type at 35°C, in contrast to mutant G268N this mutant shows no cold-adapted characteristics	Oryctolagus cuniculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphocreatine	-	Oryctolagus cuniculus	ATP + creatine	-	?
dADP + phosphocreatine	-	Oryctolagus cuniculus	?	-	?

Synonyms

Synonyms	Comment	Organism
creatinine kinase	-	Oryctolagus cuniculus

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Release 2023.1 (January 2023)