Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.3.2 extracted from

  • Iwanami, K.; Uda, K.; Tada, H.; Suzuki, T.
    Cytoplasmic and mitochondrial creatine kinases from the skeletal muscle of sperm whale (Physeter macrocephalus). Molecular cloning and enzyme characterization (2008), Protein J., 27, 43-49.
    View publication on PubMed
Search for more literature for 2.7.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a fusion protein with maltose-binding protein, mitochondrial isoform sMiCK Physeter catodon
expressed in Escherichia coli as a fusion protein with maltose-binding protein, muscle-type isoform MCK Physeter catodon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.01
-
 ATP
-
 Physeter catodon
4.3
-
 Creatine mitochondrial isoform sMiCK Physeter catodon
9.38
-
 Creatine
-
 Physeter catodon
9.38
-
 ATP mitochondrial isoform sMiCK Physeter catodon

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytoplasmic, muscle-type isoform MCK Physeter catodon 5829
-
mitochondrion sarcomeric, mitochondrial isoform sMiCK Physeter catodon 5739
-

Organism

Organism UniProt Comment Textmining
Physeter catodon
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Physeter catodon
-
skeletal muscle sMiCK Physeter catodon
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphocreatine synergistic substrate binding, mitochondrial isoform sMiCK Physeter catodon ATP + creatine
-
 ?
ADP + phosphocreatine synergistic substrate binding, muscle-type isoform MCK Physeter catodon ATP + creatine
-
 ?

Synonyms

Synonyms Comment Organism
MCK
-
 Physeter catodon
sMiCK
-
 Physeter catodon

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
36.4
-
 ATP muscle-type isoform MCK Physeter catodon
36.4
-
 Creatine muscle-type isoform MCK Physeter catodon
38.4
-
 ATP mitochondrial isoform sMiCK Physeter catodon
38.4
-
 Creatine mitochondrial isoform sMiCK Physeter catodon