Cloned (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
C146S | enzyme preparation contains only reduced form | Oryctolagus cuniculus |
C254S | enzyme preparation contains both oxidized and reduced forms | Oryctolagus cuniculus |
C283S | enzyme preparation contains both oxidized and reduced forms | Oryctolagus cuniculus |
C74S | enzyme preparation contains only reduced form | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
ATP | reduced form of creatine kinase | Oryctolagus cuniculus | |
0.38 | - |
ATP | wild-type containing both oxidized and reduced form | Oryctolagus cuniculus | |
0.43 | - |
ATP | oxidized form of creatine kinase | Oryctolagus cuniculus | |
8.33 | - |
Creatine | reduced form of creatine kinase | Oryctolagus cuniculus | |
8.39 | - |
Creatine | wild-type containing both oxidized and reduced form | Oryctolagus cuniculus | |
8.48 | - |
Creatine | oxidized form of creatine kinase | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | intrachain disulfide bond in oxidized form of enzyme is formed between C74 and C146 | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | oxidized form of enzyme | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
210 | - |
oxidized form of creatine kinase | Oryctolagus cuniculus |
261 | - |
wild-type containing both oxidized and reduced form | Oryctolagus cuniculus |
274.9 | - |
reduced form of creatine kinase | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | - |
Oryctolagus cuniculus | ADP + creatine phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | tertiary structure of oxidized form of enzyme is more easily disturbed than reduced form. The oxidized form, unlike reduced form, cannot interact with the M-line protein myomesin and therefore might have no role in muscle contraction. Oxidized creatine kinase can be rapidly ubiquitinated by muscle ring finger protein I and may be substrate of the ATP-ubiquitin-proteasome pathway | Oryctolagus cuniculus |