Literature summary for 2.7.3.2 extracted from

Hoffman, G.G.; Sona, S.; Bertin, M.; Ellington, W.R.
The role of an absolutely conserved tryptophan residue in octamer formation and stability in mitochondrial creatine kinases (2006), Biochim. Biophys. Acta, 1764, 1512-1517.

Search for more literature for 2.7.3.2

Protein Variants

Protein Variants	Comment	Organism
additional information	the enzyme from Tethya aurantia lacks the absoluteley conserved Trp residue, Trp264 in chicken sarcomeric enzyme, involved in octamer stability in most organisms and consequently forms dimers. Mutation of Tyr residue in the site corresponding to the conserved Trp in other organisms to Trp, His or Asn also yields dimers	Tethya aurantium
additional information	the Trp residue corresponding to Trp264 in chicken sarcomeric enzyme is absoluteley conserved in most organisms and involved in octamer stability. Mutation of this Trp residue to Cys, Tyr, His, Asn, or Phe produces only dimers	Chaetopterus variopedatus
W264C	absoluteley conserved residue involved in octamer stability in most organisms, mutant forms dimers instead of octamers	Gallus gallus
W264Y	absoluteley conserved residue involved in octamer stability in most organisms, mutant forms dimers instead of octamers	Gallus gallus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Gallus gallus	5739	-
mitochondrion	-	Chaetopterus variopedatus	5739	-

Organism

Organism	UniProt	Comment	Textmining
Chaetopterus variopedatus	-	-	-
Gallus gallus	-	-	-
Tethya aurantium	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
sarcoma cell	-	Gallus gallus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
22	-	mutant Trp to Phe	Chaetopterus variopedatus
26	-	mutant Trp to Asn	Chaetopterus variopedatus
26.8	-	mutant Tyr to Asn	Tethya aurantium
28.8	-	mutant Tyr to His	Tethya aurantium
29.6	-	mutant Trp to Cys	Chaetopterus variopedatus
31.8	-	wild-type	Tethya aurantium
32	-	mutant Trp to His	Chaetopterus variopedatus
36.2	-	wild-type	Chaetopterus variopedatus
36.4	-	mutant Trp to Tyr	Chaetopterus variopedatus
43.7	-	mutant W264C	Gallus gallus
62.3	-	mutant W264Y	Gallus gallus
70.2	-	wild-type	Gallus gallus
71.2	-	mutant Tyr to Trp	Tethya aurantium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphocreatine	-	Gallus gallus	ATP + creatine	-	?
ADP + phosphocreatine	-	Tethya aurantium	ATP + creatine	-	?
ADP + phosphocreatine	-	Chaetopterus variopedatus	ATP + creatine	-	?

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Release 2023.1 (January 2023)