Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I69A | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
I69L | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
I69V | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
V325A | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme, mutant shows a slight preference for cyclocreatine, i.e. 1-carboxymethy-2-iminoimidazolidine, as substrate | Homo sapiens |
V325E | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme, mutant shows a more than 100fold higher preference for N-ethylglycocyamine as substrate compared to creatine, highly reduced activity with other substrates compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, recombinant wild-type and mutant enzymes | Homo sapiens | |
0.22 | - |
ATP | pH 9.0, 30°C, recombinant wild-type enzyme, with substrate creatine | Homo sapiens | |
0.22 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
0.29 | - |
ATP | pH 9.0, 30°C, recombinant wild-type enzyme, with substrate cyclocreatine | Homo sapiens | |
0.29 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
0.62 | - |
ATP | pH 9.0, 30°C, recombinant wild-type enzyme, with substrate N-ethylglycocyamine | Homo sapiens | |
0.62 | - |
ATP | ATP in form of MgATP2- | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | Homo sapiens | - |
ADP + phosphocreatine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P12277 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + creatine = ADP + phosphocreatine | catalytic mechanism and substrate binding involving 2 flexible loops and a specificity pocket formed by Ile69 and Val325 determining substrate specificity, active site structure | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificities of recombinant wild-type and mutant enzymes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | - |
Homo sapiens | ADP + phosphocreatine | - |
r | |
ATP + cyclocreatine | i.e. 1-carboxymethyl-2-iminoimidazolidine | Homo sapiens | ADP + phospho-cyclocreatine | - |
? | |
ATP + glycocyamine | very low activity | Homo sapiens | ADP + glycocyamine phosphate | - |
? | |
ATP + N-ethylglycocyamine | - |
Homo sapiens | ADP + N-ethylglycocyamine phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the phosphagen kinase superfamily of enzymes | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
N-ethylglycocyamine | pH 9.0, 30°C, recombinant mutant I69L | Homo sapiens | |
1.5 | - |
cyclocreatine | pH 9.0, 30°C, recombinant mutant I69L | Homo sapiens | |
4.2 | - |
Creatine | pH 9.0, 30°C, recombinant mutant I69A | Homo sapiens | |
4.3 | - |
N-ethylglycocyamine | pH 9.0, 30°C, recombinant mutant V325A | Homo sapiens | |
4.4 | - |
cyclocreatine | pH 9.0, 30°C, recombinant mutant I69V | Homo sapiens | |
12 | - |
N-ethylglycocyamine | pH 9.0, 30°C, recombinant mutant I69V | Homo sapiens | |
14.5 | - |
N-ethylglycocyamine | pH 9.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
15.4 | - |
Creatine | pH 9.0, 30°C, recombinant mutant V325A | Homo sapiens | |
18 | - |
cyclocreatine | pH 9.0, 30°C, recombinant mutant V325A | Homo sapiens | |
35.2 | - |
cyclocreatine | pH 9.0, 30°C, recombinant wild-type enzyme | Homo sapiens | |
45 | - |
Creatine | pH 9.0, 30°C, recombinant mutant I69L | Homo sapiens | |
82.7 | - |
Creatine | pH 9.0, 30°C, recombinant mutant I69V | Homo sapiens | |
85.3 | - |
Creatine | pH 9.0, 30°C, recombinant wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
MgADP- | - |
Homo sapiens | |
MgATP2- | - |
Homo sapiens |