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Literature summary for 2.7.3.2 extracted from
- Purification of mitochondrial creatine kinase. Biochemical and immunological characterization (1980), J. Biol. Chem., 255, 2870-2877.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Canis lupus familiaris | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
2 * 41000, SDS-PAGE | Canis lupus familiaris |
| 84000 | - |
gel filtration | Canis lupus familiaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Canis lupus familiaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Canis lupus familiaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Canis lupus familiaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 0.05 M Tris/barbital buffer, pH 7.8, 0.01 M 2-mercaptoethanol, 17% loss of activity in 6 weeks | Canis lupus familiaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | - |
Canis lupus familiaris | ADP + creatine phosphate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 41000, SDS-PAGE | Canis lupus familiaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
isozyme MiMi-CK: 1 h, 15% loss of activity, 2 h, 34% loss of activity, 3 h, 66% loss of activity, isozyme BB-CK: 6 h, 32% loss of activity, isozyme MM-CK: no loss of activity | Canis lupus familiaris |
| 37 | - |
isozyme MiMi-CK: 10 min, 30% loss of activity, 20 min, 62% loss of activity, 80 min, 75% loss of activity, isozyme MM-CK: 80 min, 75% loss of activity | Canis lupus familiaris |
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