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Literature summary for 2.7.3.2 extracted from
- Mitochondrial creatine kinase from human heart muscle: purification and characterization of the crystallized isoenzyme (1983), J. Biochem., 94, 1247-1257.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| isozyme MiMi-CK | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
ADP | ADP in form of MgADP- | Homo sapiens |  |
| 1.7 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
| 3 | - |
creatine phosphate | - |
Homo sapiens | |
| 8 | - |
Creatine | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Gallus gallus | 5739 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
| mitochondrion | - |
Sus scrofa | 5739 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
| mitochondrion | - |
Oryctolagus cuniculus | 5739 | - |
| mitochondrion | - |
Papio anubis | 5739 | - |
| mitochondrion | - |
trout | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required as MgATP | Gallus gallus | |
| Mg2+ | required as MgATP | Homo sapiens | |
| Mg2+ | required as MgATP | Rattus norvegicus | |
| Mg2+ | required as MgATP | Sus scrofa | |
| Mg2+ | required as MgATP | Bos taurus | |
| Mg2+ | required as MgATP | Oryctolagus cuniculus | |
| Mg2+ | required as MgATP | Papio anubis | |
| Mg2+ | required as MgATP | trout | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE | Homo sapiens |
| 84000 | 85000 | isozyme MiMi-CK, sedimentation equilibrium centrifugation, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Papio anubis | - |
monkey | - |
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
| trout | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Gallus gallus | - |
| heart | - |
Homo sapiens | - |
| heart | - |
Rattus norvegicus | - |
| heart | - |
Sus scrofa | - |
| heart | - |
Bos taurus | - |
| heart | - |
Oryctolagus cuniculus | - |
| heart | - |
Papio anubis | - |
| heart | - |
trout | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15 | - |
synthesis of phosphocreatine | Homo sapiens |
| 49.5 | - |
synthesis of ATP | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 7.0, 1 mM dithiothreitol or 14 mM 2-mercaptoethanol, at least 3 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphocreatine | - |
Homo sapiens | ATP + creatine | - |
r | |
| ATP + creatine | ATP required as MgATP2- | Gallus gallus | ADP + creatine phosphate | - |
r | |
| ATP + creatine | ATP required as MgATP2- | Rattus norvegicus | ADP + creatine phosphate | - |
r | |
| ATP + creatine | ATP required as MgATP2- | Sus scrofa | ADP + creatine phosphate | - |
r | |
| ATP + creatine | ATP required as MgATP2- | Bos taurus | ADP + creatine phosphate | - |
r | |
| ATP + creatine | ATP required as MgATP2- | Oryctolagus cuniculus | ADP + creatine phosphate | - |
r | |
| ATP + creatine | ATP required as MgATP2- | Papio anubis | ADP + creatine phosphate | - |
r | |
| ATP + creatine | ATP required as MgATP2- | trout | ADP + creatine phosphate | - |
r | |
| ATP + creatine | ATP required as MgATP2- | Homo sapiens | ADP + creatine phosphate | in the reverse direction ADP can be replaced by IDP with 18% efficiency, ADP cannot be replaced by GDP, CDP, UDP, dTDP | r | |
| ATP + creatine | - |
Homo sapiens | ADP + phosphocreatine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Gallus gallus |
| dimer | - |
Rattus norvegicus |
| dimer | - |
Sus scrofa |
| dimer | - |
Bos taurus |
| dimer | - |
Oryctolagus cuniculus |
| dimer | - |
Papio anubis |
| dimer | - |
trout |
| dimer | 2 * 42000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
synthesis of MgATP2- | Homo sapiens |
| 8.7 | - |
synthesis of phosphocreatine | Gallus gallus |
| 8.7 | - |
synthesis of phosphocreatine | Homo sapiens |
| 8.7 | - |
synthesis of phosphocreatine | Rattus norvegicus |
| 8.7 | - |
synthesis of phosphocreatine | Sus scrofa |
| 8.7 | - |
synthesis of phosphocreatine | Bos taurus |
| 8.7 | - |
synthesis of phosphocreatine | Oryctolagus cuniculus |
| 8.7 | - |
synthesis of phosphocreatine | Papio anubis |
| 8.7 | - |
synthesis of phosphocreatine | trout |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10.5 | rapid inactivation above and below | Homo sapiens |
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