Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
ATP | at pH 8.0 and 30°C | Campylobacter jejuni | |
0.64 | - |
L-glutamine | at pH 8.0 and 30°C | Campylobacter jejuni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + H2O | Campylobacter jejuni | - |
AMP + phosphate + N5-phospho-L-glutamine | - |
? | |
ATP + L-glutamine + H2O | Campylobacter jejuni NCTC11168 | - |
AMP + phosphate + N5-phospho-L-glutamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Campylobacter jejuni NCTC11168 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni affinity column chromatography | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + H2O | - |
Campylobacter jejuni | AMP + phosphate + N5-phospho-L-glutamine | - |
? | |
ATP + L-glutamine + H2O | - |
Campylobacter jejuni NCTC11168 | AMP + phosphate + N5-phospho-L-glutamine | - |
? | |
additional information | no activity with ammonia, L-glutamate, or L-asparagine | Campylobacter jejuni | ? | - |
? | |
additional information | no activity with ammonia, L-glutamate, or L-asparagine | Campylobacter jejuni NCTC11168 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cj1418 | - |
Campylobacter jejuni |
L-glutamine kinase | - |
Campylobacter jejuni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
ATP | at pH 8.0 and 30°C | Campylobacter jejuni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
L-glutamine | at pH 8.0 and 30°C | Campylobacter jejuni | |
7.4 | - |
ATP | at pH 8.0 and 30°C | Campylobacter jejuni |