Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.3.13 extracted from

  • Taylor, Z.W.; Brown, H.A.; Narindoshvili, T.; Wenzel, C.Q.; Szymanski, C.M.; Holden, H.M.; Raushel, F.M.
    Discovery of a glutamine kinase required for the biosynthesis of the O-methyl phosphoramidate modifications found in the capsular polysaccharides of Campylobacter jejuni (2017), J. Am. Chem. Soc., 139, 9463-9466 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
ATP at pH 8.0 and 30°C Campylobacter jejuni
0.64
-
L-glutamine at pH 8.0 and 30°C Campylobacter jejuni

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamine + H2O Campylobacter jejuni
-
AMP + phosphate + N5-phospho-L-glutamine
-
?
ATP + L-glutamine + H2O Campylobacter jejuni NCTC11168
-
AMP + phosphate + N5-phospho-L-glutamine
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-
Campylobacter jejuni NCTC11168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni affinity column chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamine + H2O
-
Campylobacter jejuni AMP + phosphate + N5-phospho-L-glutamine
-
?
ATP + L-glutamine + H2O
-
Campylobacter jejuni NCTC11168 AMP + phosphate + N5-phospho-L-glutamine
-
?
additional information no activity with ammonia, L-glutamate, or L-asparagine Campylobacter jejuni ?
-
?
additional information no activity with ammonia, L-glutamate, or L-asparagine Campylobacter jejuni NCTC11168 ?
-
?

Synonyms

Synonyms Comment Organism
Cj1418
-
Campylobacter jejuni
L-glutamine kinase
-
Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
ATP at pH 8.0 and 30°C Campylobacter jejuni

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.9
-
L-glutamine at pH 8.0 and 30°C Campylobacter jejuni
7.4
-
ATP at pH 8.0 and 30°C Campylobacter jejuni