BRENDA - Enzyme Database show
show all sequences of 2.7.3.10

Phosphagens in protozoa - II. Presence of phosphagen kinase in Ochromonas danica

Piccinni, E.; Coppellotti, O.; Comp. Biochem. Physiol. B 62, 287-289 (1979)
No PubMed abstract available

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
no activity without divalent cation, Ca2+ is more effective than Mg2+
Ochromonas danica
Mg2+
no activity without divalent cation, less effective than Ca2+
Ochromonas danica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
gel filtration
Ochromonas danica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ochromonas danica
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + agmatine
-
642370
Ochromonas danica
ADP + N4-phosphoagmatine
-
-
-
?
ATP + L-Arg
18% of the activity with agamatine
642370
Ochromonas danica
ADP + N5-phosphoarginine
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
2 optima in the reaction with agmatine in presence of Ca2+: pH 7.5 and pH 9.0. Reaction with L-Arg occurs only at pH 7.5-8.0, activity at pH 7.5 is higher than at pH 8.0
Ochromonas danica
9
-
2 optima in the reaction with agmatine in presence of Ca2+: pH 7.5 and pH 9.0
Ochromonas danica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
8
reaction with L-Arg occurs only at pH 7.5-8.0, no activity at pH 7.0 and 8.6
Ochromonas danica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
no activity without divalent cation, Ca2+ is more effective than Mg2+
Ochromonas danica
Mg2+
no activity without divalent cation, less effective than Ca2+
Ochromonas danica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
gel filtration
Ochromonas danica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + agmatine
-
642370
Ochromonas danica
ADP + N4-phosphoagmatine
-
-
-
?
ATP + L-Arg
18% of the activity with agamatine
642370
Ochromonas danica
ADP + N5-phosphoarginine
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
2 optima in the reaction with agmatine in presence of Ca2+: pH 7.5 and pH 9.0. Reaction with L-Arg occurs only at pH 7.5-8.0, activity at pH 7.5 is higher than at pH 8.0
Ochromonas danica
9
-
2 optima in the reaction with agmatine in presence of Ca2+: pH 7.5 and pH 9.0
Ochromonas danica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
8
reaction with L-Arg occurs only at pH 7.5-8.0, no activity at pH 7.0 and 8.6
Ochromonas danica
Other publictions for EC 2.7.3.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
642370
Piccinni
-
Phosphagens in protozoa - II. ...
Ochromonas danica
Comp. Biochem. Physiol. B
62
287-289
1979
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