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Literature summary for 2.7.2.8 extracted from

  • Xu, M.; Rao, Z.; Dou, W.; Yang, J.; Jin, J.; Xu, Z.
    Site-directed mutagenesis and feedback-resistant N-acetyl-L-glutamate kinase (NAGK) increase Corynebacterium crenatum L-arginine production (2012), Amino Acids, 43, 255-266.
    View publication on PubMed
Search for more literature for 2.7.2.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Corynebacterium crenatum

Protein Variants

Protein Variants Comment Organism
E19R Km and kcat (N-acetyl-L-glutamate) similar to wild-type, IC50 (L-arginine) approximately 55fold increased compared to wild-type Corynebacterium crenatum
H268N Km and kcat (N-acetyl-L-glutamate) similar to wild-type, IC50 (L-arginine) approximately 55fold increased compared to wild-type Corynebacterium crenatum
H268N/H26E Km and kcat (N-acetyl-L-glutamate) similar to wild-type, IC50 (L-arginine) 875fold increased compared to wild-type Corynebacterium crenatum
H268N/H26E/E19R Km and kcat (N-acetyl-L-glutamate) similar to wild-type, IC50 (L-arginine) 1960fold increased compared to wild-type Corynebacterium crenatum
H26E Km and kcat (N-acetyl-L-glutamate) similar to wild-type, IC50 (L-arginine) approximately 55fold increased compared to wild-type Corynebacterium crenatum

Inhibitors

Inhibitors Comment Organism Structure
L-arginine
-
 Corynebacterium crenatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.29
-
 N-acetyl-L-glutamate mutant H26E, pH 8.0, 37°C Corynebacterium crenatum
3.33
-
 N-acetyl-L-glutamate mutant E19R, pH 8.0, 37°C Corynebacterium crenatum
3.36
-
 N-acetyl-L-glutamate mutant H268N/H26E/E19R, pH 8.0, 37°C Corynebacterium crenatum
3.42
-
 N-acetyl-L-glutamate wild-type, pH 8.0, 37°C Corynebacterium crenatum
3.42
-
 N-acetyl-L-glutamate mutant H268N/H26E, pH 8.0, 37°C Corynebacterium crenatum
3.45
-
 N-acetyl-L-glutamate mutant H268N, pH 8.0, 37°C Corynebacterium crenatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 recombinant protein Corynebacterium crenatum

Organism

Organism UniProt Comment Textmining
Corynebacterium crenatum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-glutamate
-
 Corynebacterium crenatum ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
argB
-
 Corynebacterium crenatum
NagK
-
 Corynebacterium crenatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Corynebacterium crenatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31.4
-
 N-acetyl-L-glutamate wild-type, pH 8.0, 37°C, with 1 mM L-arginine Corynebacterium crenatum
41
-
 N-acetyl-L-glutamate mutant H26E, pH 8.0, 37°C, with 1 mM L-arginine Corynebacterium crenatum
41.1
-
 N-acetyl-L-glutamate mutant H268N, pH 8.0, 37°C, with 1 mM L-arginine Corynebacterium crenatum
42
-
 N-acetyl-L-glutamate mutant E19R, pH 8.0, 37°C, with 1 mM L-arginine Corynebacterium crenatum
44.4
-
 N-acetyl-L-glutamate mutant H26E, pH 8.0, 37°C Corynebacterium crenatum
45.1
-
 N-acetyl-L-glutamate wild-type, pH 8.0, 37°C Corynebacterium crenatum
45.3
-
 N-acetyl-L-glutamate mutant H268N, pH 8.0, 37°C Corynebacterium crenatum
45.6
-
 N-acetyl-L-glutamate mutant H268N/H26E, pH 8.0, 37°C, with 1 mM L-arginine Corynebacterium crenatum
45.8
-
 N-acetyl-L-glutamate mutant E19R, pH 8.0, 37°C Corynebacterium crenatum
46
-
 N-acetyl-L-glutamate mutant H268N/H26E/E19R, pH 8.0, 37°C, with 1 mM L-arginine Corynebacterium crenatum
46.1
-
 N-acetyl-L-glutamate mutant H268N/H26E, pH 8.0, 37°C Corynebacterium crenatum
46.5
-
 N-acetyl-L-glutamate mutant H268N/H26E/E19R, pH 8.0, 37°C Corynebacterium crenatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Corynebacterium crenatum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.4
-
 wild-type, pH 8.0, 37°C Corynebacterium crenatum L-arginine
21
-
 mutant H26E, pH 8.0, 37°C Corynebacterium crenatum L-arginine
22
-
 mutant H268N, pH 8.0, 37°C Corynebacterium crenatum L-arginine
24
-
 mutant E19R, pH 8.0, 37°C Corynebacterium crenatum L-arginine
350
-
 mutant H268N/H26E, pH 8.0, 37°C Corynebacterium crenatum L-arginine
784
-
 mutant H268N/H26E/E19R, pH 8.0, 37°C Corynebacterium crenatum L-arginine

General Information

General Information Comment Organism
malfunction argB gene coding the N-acetyl-L-glutamate kinase is first overexpressed in the strain SYPA5-5, whereas the L-arginine production is narrowly increased by 15.4% Corynebacterium crenatum