BRENDA - Enzyme Database show
show all sequences of 2.7.2.7

Crystal structure of butyrate kinase 2 from Thermotoga maritima, a member of the ASKHA superfamily of phosphotransferases

Diao, J.; Hasson, M.S.; J. Bacteriol. 191, 2521-2529 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli B834 cells
Thermotoga maritima
Crystallization (Commentary)
Crystallization
Organism
Buk2 complexed with (beta,gamma-methylene) adenosine 5'-triphosphate, sitting drop vapor diffusion method, using 1.7-3.0 M sodium formate, 1 M acetic acid (pH 4.5)
Thermotoga maritima
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + butanoate
Thermotoga maritima
-
ADP + butanoyl phosphate
-
-
r
ATP + isobutanoate
Thermotoga maritima
-
ADP + isobutanoyl phosphate
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermotoga maritima
Q9X278
-
-
Purification (Commentary)
Commentary
Organism
-
Thermotoga maritima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + butanoate
-
704285
Thermotoga maritima
ADP + butanoyl phosphate
-
-
-
r
ATP + isobutanoate
-
704285
Thermotoga maritima
ADP + isobutanoyl phosphate
-
-
-
r
Subunits
Subunits
Commentary
Organism
octamer
x-ray crystallography
Thermotoga maritima
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Thermotoga maritima
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli B834 cells
Thermotoga maritima
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Thermotoga maritima
Crystallization (Commentary) (protein specific)
Crystallization
Organism
Buk2 complexed with (beta,gamma-methylene) adenosine 5'-triphosphate, sitting drop vapor diffusion method, using 1.7-3.0 M sodium formate, 1 M acetic acid (pH 4.5)
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + butanoate
Thermotoga maritima
-
ADP + butanoyl phosphate
-
-
r
ATP + isobutanoate
Thermotoga maritima
-
ADP + isobutanoyl phosphate
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + butanoate
-
704285
Thermotoga maritima
ADP + butanoyl phosphate
-
-
-
r
ATP + isobutanoate
-
704285
Thermotoga maritima
ADP + isobutanoyl phosphate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
octamer
x-ray crystallography
Thermotoga maritima
Other publictions for EC 2.7.2.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703499
Sullivan
-
Structural correlations of act ...
Clostridium acetobutylicum
Enzyme Microb. Technol.
46
118-124
2010
-
-
-
-
14
-
-
-
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-
1
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1
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1
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1
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1
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1
-
14
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1
-
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-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704285
Diao
Crystal structure of butyrate ...
Thermotoga maritima
J. Bacteriol.
191
2521-2529
2009
-
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1
1
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2
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2
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1
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2
1
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1
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1
1
1
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2
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1
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-
2
1
-
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-
-
-
-
-
-
-
-
-
-
-
706668
Diao
Open and closed conformations ...
Thermotoga maritima
Proteins
80
1712
2009
-
-
-
1
-
-
-
-
-
-
-
2
-
2
-
-
1
-
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2
1
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1
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-
1
1
-
-
-
-
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-
2
-
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-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691411
Senger
Genome-scale model for Clostri ...
Clostridium acetobutylicum
Biotechnol. Bioeng.
101
1036-1052
2008
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
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1
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693994
Sillers
Metabolic engineering of the n ...
Clostridium acetobutylicum, Clostridium acetobutylicum M5
Metab. Eng.
10
321-332
2008
-
1
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
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-
1
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-
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-
-
-
-
-
-
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-
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-
677610
Paillard
Relation between phylogenetic ...
Butyrivibrio fibrisolvens, Butyrivibrio fibrisolvens ATCC 19171 / D1, Butyrivibrio hungatei, Butyrivibrio hungatei JK615T, Butyrivibrio proteoclasticus, Butyrivibrio proteoclasticus B316T
Antonie van Leeuwenhoek
91
417-422
2007
-
-
3
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
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-
-
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-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681800
Tessmer
Heat-shock protein HspA mimics ...
Clostridium acetobutylicum
Microbiology
153
366-374
2007
-
2
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691537
Raz
Isolation and characterisation ...
Enterococcus durans
Br. J. Nutr.
97
725-734
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679539
Liu
-
Butyric acid and hydrogen prod ...
Clostridium tyrobutyricum
Enzyme Microb. Technol.
38
521-528
2006
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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1
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-
-
1
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-
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-
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-
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-
-
-
486475
Liu
Exploitation of butyrate kinas ...
Clostridium acetobutylicum
Appl. Microbiol. Biotechnol.
53
545-552
2000
-
1
-
-
-
-
-
-
-
-
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-
4
-
-
1
-
-
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1
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1
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1
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1
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-
642349
Huang
Identification and characteriz ...
Clostridium acetobutylicum
J. Mol. Microbiol. Biotechnol.
2
33-38
2000
-
-
1
-
-
-
-
1
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
1
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-
1
1
-
-
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-
-
1
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-
-
-
-
-
-
1
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-
2
-
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1
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
486471
Cary
Cloning and expression of Clos ...
Clostridium acetobutylicum
J. Bacteriol.
170
4613-4618
1988
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
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-
-
-
-
-
1
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1
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1
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-
-
642344
Hartmanis
Butyrate kinase from Clostridi ...
Clostridium acetobutylicum
J. Biol. Chem.
262
617-621
1987
1
-
-
-
-
-
1
2
-
2
2
-
-
2
-
-
1
-
-
-
1
1
7
1
-
-
2
-
1
1
1
-
-
1
-
1
-
-
-
-
-
-
-
1
-
2
-
2
2
-
-
-
-
1
-
-
1
1
7
1
-
-
2
-
1
1
1
1
-
-
-
-
-
-
642345
Ballongue
-
Regulation of acetate kinase a ...
Clostridium acetobutylicum
FEMS Microbiol. Lett.
35
295-301
1986
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
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1
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2
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
286050
Yarlett
Butyrate formation from glucos ...
Dasytricha ruminantium
Biochem. J.
228
187-192
1985
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
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1
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2
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1
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1
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1
-
2
-
-
-
-
-
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-
-
-
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-
-
-
-
642348
Twarog
Role of butyryl phosphate in t ...
Clostridium butyricum, Clostridium pasteurianum, Clostridium tetanomorphum, Clostridium tyrobutyricum
J. Bacteriol.
86
112-117
1963
-
-
-
-
-
-
-
7
-
2
-
-
-
4
-
-
1
-
-
-
1
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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7
-
2
-
-
-
-
-
1
-
-
1
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
642343
Twarog
Enzymic phosphorylation of but ...
Clostridium butyricum
J. Biol. Chem.
237
2474-2477
1962
-
-
-
-
-
-
2
4
-
5
-
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-
1
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-
-
-
-
-
-
-
5
-
-
-
-
-
1
1
-
-
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-
-
-
-
-
-
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-
-
-
2
-
4
-
5
-
-
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5
-
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-
1
1
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