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show all sequences of 2.7.2.7

Structural correlations of activity of Clostridium acetobutylicum ATCC 824 butyrate kinase isozymes

Sullivan, L.; Cates, M.; Bennett, G.; Enzyme Microb. Technol. 46, 118-124 (2010)
No PubMed abstract available

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
E332D
the mutant shows 3.82% of wild type butyrate kinase II activity
Clostridium acetobutylicum
E332L
the mutant shows 0.34% of wild type butyrate kinase II activity
Clostridium acetobutylicum
E332Q
the mutant shows 3.02% of wild type butyrate kinase II activity
Clostridium acetobutylicum
E334D
the mutant shows 0.29% of wild type butyrate kinase II activity
Clostridium acetobutylicum
E334L
the mutant shows0.58% of wild type butyrate kinase II activity
Clostridium acetobutylicum
E334Q
the mutant shows 0.41% of wild type butyrate kinase II activity
Clostridium acetobutylicum
H155L
the mutant shows no butyrate kinase II activity
Clostridium acetobutylicum
H155Q
the mutant shows no butyrate kinase II activity
Clostridium acetobutylicum
H183L
the mutant shows 0.06% of wild type butyrate kinase II activity
Clostridium acetobutylicum
H183Q
the mutant shows 0.19% of wild type butyrate kinase II activity
Clostridium acetobutylicum
R150F
the mutant shows no butyrate kinase II activity
Clostridium acetobutylicum
R150K
the mutant shows 21% of wild type butyrate kinase II activity
Clostridium acetobutylicum
R215F E332D
the mutant shows 0.38% of wild type butyrate kinase II activity
Clostridium acetobutylicum
R215K
the mutant shows 0.44% of wild type butyrate kinase II activity
Clostridium acetobutylicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
SDS-PAGE
Clostridium acetobutylicum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium acetobutylicum
-
strain ATCC 824
-
Purification (Commentary)
Commentary
Organism
nickel affinity column chromatography
Clostridium acetobutylicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + butanoate
-
703499
Clostridium acetobutylicum
ADP + butanoyl phosphate
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Clostridium acetobutylicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Clostridium acetobutylicum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E332D
the mutant shows 3.82% of wild type butyrate kinase II activity
Clostridium acetobutylicum
E332L
the mutant shows 0.34% of wild type butyrate kinase II activity
Clostridium acetobutylicum
E332Q
the mutant shows 3.02% of wild type butyrate kinase II activity
Clostridium acetobutylicum
E334D
the mutant shows 0.29% of wild type butyrate kinase II activity
Clostridium acetobutylicum
E334L
the mutant shows0.58% of wild type butyrate kinase II activity
Clostridium acetobutylicum
E334Q
the mutant shows 0.41% of wild type butyrate kinase II activity
Clostridium acetobutylicum
H155L
the mutant shows no butyrate kinase II activity
Clostridium acetobutylicum
H155Q
the mutant shows no butyrate kinase II activity
Clostridium acetobutylicum
H183L
the mutant shows 0.06% of wild type butyrate kinase II activity
Clostridium acetobutylicum
H183Q
the mutant shows 0.19% of wild type butyrate kinase II activity
Clostridium acetobutylicum
R150F
the mutant shows no butyrate kinase II activity
Clostridium acetobutylicum
R150K
the mutant shows 21% of wild type butyrate kinase II activity
Clostridium acetobutylicum
R215F E332D
the mutant shows 0.38% of wild type butyrate kinase II activity
Clostridium acetobutylicum
R215K
the mutant shows 0.44% of wild type butyrate kinase II activity
Clostridium acetobutylicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
SDS-PAGE
Clostridium acetobutylicum
Purification (Commentary) (protein specific)
Commentary
Organism
nickel affinity column chromatography
Clostridium acetobutylicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + butanoate
-
703499
Clostridium acetobutylicum
ADP + butanoyl phosphate
-
-
-
r
Other publictions for EC 2.7.2.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703499
Sullivan
-
Structural correlations of act ...
Clostridium acetobutylicum
Enzyme Microb. Technol.
46
118-124
2010
-
-
-
-
14
-
-
-
-
-
1
-
-
1
-
-
1
-
-
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-
1
-
-
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-
-
-
-
-
1
-
-
-
-
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-
1
-
14
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704285
Diao
Crystal structure of butyrate ...
Thermotoga maritima
J. Bacteriol.
191
2521-2529
2009
-
-
1
1
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706668
Diao
Open and closed conformations ...
Thermotoga maritima
Proteins
80
1712
2009
-
-
-
1
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
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-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691411
Senger
Genome-scale model for Clostri ...
Clostridium acetobutylicum
Biotechnol. Bioeng.
101
1036-1052
2008
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
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1
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1
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-
-
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
693994
Sillers
Metabolic engineering of the n ...
Clostridium acetobutylicum, Clostridium acetobutylicum M5
Metab. Eng.
10
321-332
2008
-
1
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
1
1
-
-
1
-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677610
Paillard
Relation between phylogenetic ...
Butyrivibrio fibrisolvens, Butyrivibrio fibrisolvens ATCC 19171 / D1, Butyrivibrio hungatei, Butyrivibrio hungatei JK615T, Butyrivibrio proteoclasticus, Butyrivibrio proteoclasticus B316T
Antonie van Leeuwenhoek
91
417-422
2007
-
-
3
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681800
Tessmer
Heat-shock protein HspA mimics ...
Clostridium acetobutylicum
Microbiology
153
366-374
2007
-
2
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691537
Raz
Isolation and characterisation ...
Enterococcus durans
Br. J. Nutr.
97
725-734
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
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-
-
-
-
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-
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1
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-
-
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-
1
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-
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-
-
-
-
-
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-
-
-
-
-
-
-
-
-
679539
Liu
-
Butyric acid and hydrogen prod ...
Clostridium tyrobutyricum
Enzyme Microb. Technol.
38
521-528
2006
-
-
-
-
1
-
-
-
-
-
-
-
-
1
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-
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486475
Liu
Exploitation of butyrate kinas ...
Clostridium acetobutylicum
Appl. Microbiol. Biotechnol.
53
545-552
2000
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
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1
-
-
-
-
-
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-
-
-
-
-
-
-
1
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-
-
-
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-
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-
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-
-
1
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642349
Huang
Identification and characteriz ...
Clostridium acetobutylicum
J. Mol. Microbiol. Biotechnol.
2
33-38
2000
-
-
1
-
-
-
-
1
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
486471
Cary
Cloning and expression of Clos ...
Clostridium acetobutylicum
J. Bacteriol.
170
4613-4618
1988
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
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1
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-
-
1
-
-
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-
-
-
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-
-
-
-
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-
-
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642344
Hartmanis
Butyrate kinase from Clostridi ...
Clostridium acetobutylicum
J. Biol. Chem.
262
617-621
1987
1
-
-
-
-
-
1
2
-
2
2
-
-
2
-
-
1
-
-
-
1
1
7
1
-
-
2
-
1
1
1
-
-
1
-
1
-
-
-
-
-
-
-
1
-
2
-
2
2
-
-
-
-
1
-
-
1
1
7
1
-
-
2
-
1
1
1
1
-
-
-
-
-
-
642345
Ballongue
-
Regulation of acetate kinase a ...
Clostridium acetobutylicum
FEMS Microbiol. Lett.
35
295-301
1986
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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-
-
-
-
-
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2
-
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1
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2
-
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-
-
-
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-
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-
286050
Yarlett
Butyrate formation from glucos ...
Dasytricha ruminantium
Biochem. J.
228
187-192
1985
-
-
-
-
-
-
-
-
1
-
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1
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2
-
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1
-
2
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1
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1
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1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642348
Twarog
Role of butyryl phosphate in t ...
Clostridium butyricum, Clostridium pasteurianum, Clostridium tetanomorphum, Clostridium tyrobutyricum
J. Bacteriol.
86
112-117
1963
-
-
-
-
-
-
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7
-
2
-
-
-
4
-
-
1
-
-
-
1
-
8
-
-
-
-
-
2
-
-
-
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-
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7
-
2
-
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1
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1
-
8
-
-
-
-
-
2
-
-
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-
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642343
Twarog
Enzymic phosphorylation of but ...
Clostridium butyricum
J. Biol. Chem.
237
2474-2477
1962
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-
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2
4
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5
-
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1
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5
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1
1
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2
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4
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5
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5
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1
1
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