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Literature summary for 2.7.2.7 extracted from

  • Sullivan, L.; Cates, M.; Bennett, G.
    Structural correlations of activity of Clostridium acetobutylicum ATCC 824 butyrate kinase isozymes (2010), Enzyme Microb. Technol., 46, 118-124.
No PubMed abstract available
Search for more literature for 2.7.2.7

Protein Variants

Protein Variants Comment Organism
E332D the mutant shows 3.82% of wild type butyrate kinase II activity Clostridium acetobutylicum
E332L the mutant shows 0.34% of wild type butyrate kinase II activity Clostridium acetobutylicum
E332Q the mutant shows 3.02% of wild type butyrate kinase II activity Clostridium acetobutylicum
E334D the mutant shows 0.29% of wild type butyrate kinase II activity Clostridium acetobutylicum
E334L the mutant shows0.58% of wild type butyrate kinase II activity Clostridium acetobutylicum
E334Q the mutant shows 0.41% of wild type butyrate kinase II activity Clostridium acetobutylicum
H155L the mutant shows no butyrate kinase II activity Clostridium acetobutylicum
H155Q the mutant shows no butyrate kinase II activity Clostridium acetobutylicum
H183L the mutant shows 0.06% of wild type butyrate kinase II activity Clostridium acetobutylicum
H183Q the mutant shows 0.19% of wild type butyrate kinase II activity Clostridium acetobutylicum
R150F the mutant shows no butyrate kinase II activity Clostridium acetobutylicum
R150K the mutant shows 21% of wild type butyrate kinase II activity Clostridium acetobutylicum
R215F E332D the mutant shows 0.38% of wild type butyrate kinase II activity Clostridium acetobutylicum
R215K the mutant shows 0.44% of wild type butyrate kinase II activity Clostridium acetobutylicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 SDS-PAGE Clostridium acetobutylicum

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
-
 strain ATCC 824
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + butanoate
-
 Clostridium acetobutylicum ADP + butanoyl phosphate
-
 r

Synonyms

Synonyms Comment Organism
BKI isozyme Clostridium acetobutylicum
BKII isozyme Clostridium acetobutylicum
butyrate kinase I isozyme Clostridium acetobutylicum
butyrate kinase II isozyme Clostridium acetobutylicum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Clostridium acetobutylicum