BRENDA - Enzyme Database show
show all sequences of 2.7.2.7

Role of butyryl phosphate in the energy metabolism of Clostridium tetanomorphum

Twarog, R.; Wolfe, R.S.; J. Bacteriol. 86, 112-117 (1963)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.23
-
ATP
pH 7.5, 30°C, reaction with butanoate, activation with 6 mM MgSO4
Clostridium tetanomorphum
5.06
-
ATP
pH 7.5, 30°C, reaction with butanoate, activation with 6 mM MnSO4
Clostridium tetanomorphum
10.8
-
Butanoate
pH 7.5, 30°C, activation with 10 mM MgSO4
Clostridium tetanomorphum
14.3
-
Butanoate
pH 7.5, 30°C, activation with 6 mM MnSO4
Clostridium tetanomorphum
15.3
-
valerate
pH 7.5, 30°C, activation with 6 mM MnSO4
Clostridium tetanomorphum
15.5
-
valerate
pH 7.5, 30°C, activation with 10 mM MgSO4
Clostridium tetanomorphum
77
-
Isobutanoate
pH 7.5, 30°C, activation with 10 mM MgSO4
Clostridium tetanomorphum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Clostridium butyricum
Mg2+
divalent cation requirement is satisfied by Mg2+
Clostridium tetanomorphum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium butyricum
-
-
-
Clostridium pasteurianum
-
-
-
Clostridium tetanomorphum
-
-
-
Clostridium tyrobutyricum
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Clostridium tetanomorphum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
64.3
-
-
Clostridium tetanomorphum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + butanoate
-
642348
Clostridium butyricum
ADP + butanoyl phosphate
-
-
-
-
ATP + butanoate
-
642348
Clostridium tetanomorphum
ADP + butanoyl phosphate
-
-
-
?
ATP + isobutanoate
-
642348
Clostridium butyricum
ADP + isobutanoyl phosphate
-
-
-
?
ATP + isobutanoate
-
642348
Clostridium tetanomorphum
ADP + isobutanoyl phosphate
-
-
-
?
ATP + propionate
-
642348
Clostridium butyricum
ADP + propanoyl phosphate
-
-
-
-
ATP + propionate
-
642348
Clostridium tetanomorphum
ADP + propanoyl phosphate
-
-
-
?
ATP + valerate
-
642348
Clostridium butyricum
ADP + pentanoyl phosphate
-
-
-
-
ATP + valerate
-
642348
Clostridium tetanomorphum
ADP + pentanoyl phosphate
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
8.3
activation by Mg2+
Clostridium tetanomorphum
7.5
7.6
activation by Mn2+
Clostridium tetanomorphum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.23
-
ATP
pH 7.5, 30°C, reaction with butanoate, activation with 6 mM MgSO4
Clostridium tetanomorphum
5.06
-
ATP
pH 7.5, 30°C, reaction with butanoate, activation with 6 mM MnSO4
Clostridium tetanomorphum
10.8
-
Butanoate
pH 7.5, 30°C, activation with 10 mM MgSO4
Clostridium tetanomorphum
14.3
-
Butanoate
pH 7.5, 30°C, activation with 6 mM MnSO4
Clostridium tetanomorphum
15.3
-
valerate
pH 7.5, 30°C, activation with 6 mM MnSO4
Clostridium tetanomorphum
15.5
-
valerate
pH 7.5, 30°C, activation with 10 mM MgSO4
Clostridium tetanomorphum
77
-
Isobutanoate
pH 7.5, 30°C, activation with 10 mM MgSO4
Clostridium tetanomorphum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Clostridium butyricum
Mg2+
divalent cation requirement is satisfied by Mg2+
Clostridium tetanomorphum
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Clostridium tetanomorphum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
64.3
-
-
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + butanoate
-
642348
Clostridium butyricum
ADP + butanoyl phosphate
-
-
-
-
ATP + butanoate
-
642348
Clostridium tetanomorphum
ADP + butanoyl phosphate
-
-
-
?
ATP + isobutanoate
-
642348
Clostridium butyricum
ADP + isobutanoyl phosphate
-
-
-
?
ATP + isobutanoate
-
642348
Clostridium tetanomorphum
ADP + isobutanoyl phosphate
-
-
-
?
ATP + propionate
-
642348
Clostridium butyricum
ADP + propanoyl phosphate
-
-
-
-
ATP + propionate
-
642348
Clostridium tetanomorphum
ADP + propanoyl phosphate
-
-
-
?
ATP + valerate
-
642348
Clostridium butyricum
ADP + pentanoyl phosphate
-
-
-
-
ATP + valerate
-
642348
Clostridium tetanomorphum
ADP + pentanoyl phosphate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
8.3
activation by Mg2+
Clostridium tetanomorphum
7.5
7.6
activation by Mn2+
Clostridium tetanomorphum
Other publictions for EC 2.7.2.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703499
Sullivan
-
Structural correlations of act ...
Clostridium acetobutylicum
Enzyme Microb. Technol.
46
118-124
2010
-
-
-
-
14
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
14
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704285
Diao
Crystal structure of butyrate ...
Thermotoga maritima
J. Bacteriol.
191
2521-2529
2009
-
-
1
1
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706668
Diao
Open and closed conformations ...
Thermotoga maritima
Proteins
80
1712
2009
-
-
-
1
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691411
Senger
Genome-scale model for Clostri ...
Clostridium acetobutylicum
Biotechnol. Bioeng.
101
1036-1052
2008
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693994
Sillers
Metabolic engineering of the n ...
Clostridium acetobutylicum, Clostridium acetobutylicum M5
Metab. Eng.
10
321-332
2008
-
1
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677610
Paillard
Relation between phylogenetic ...
Butyrivibrio fibrisolvens, Butyrivibrio fibrisolvens ATCC 19171 / D1, Butyrivibrio hungatei, Butyrivibrio hungatei JK615T, Butyrivibrio proteoclasticus, Butyrivibrio proteoclasticus B316T
Antonie van Leeuwenhoek
91
417-422
2007
-
-
3
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681800
Tessmer
Heat-shock protein HspA mimics ...
Clostridium acetobutylicum
Microbiology
153
366-374
2007
-
2
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691537
Raz
Isolation and characterisation ...
Enterococcus durans
Br. J. Nutr.
97
725-734
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679539
Liu
-
Butyric acid and hydrogen prod ...
Clostridium tyrobutyricum
Enzyme Microb. Technol.
38
521-528
2006
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
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-
-
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-
-
1
-
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-
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-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486475
Liu
Exploitation of butyrate kinas ...
Clostridium acetobutylicum
Appl. Microbiol. Biotechnol.
53
545-552
2000
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
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-
-
-
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-
-
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-
-
1
-
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-
1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642349
Huang
Identification and characteriz ...
Clostridium acetobutylicum
J. Mol. Microbiol. Biotechnol.
2
33-38
2000
-
-
1
-
-
-
-
1
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
486471
Cary
Cloning and expression of Clos ...
Clostridium acetobutylicum
J. Bacteriol.
170
4613-4618
1988
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
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1
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1
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1
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-
-
-
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-
-
-
642344
Hartmanis
Butyrate kinase from Clostridi ...
Clostridium acetobutylicum
J. Biol. Chem.
262
617-621
1987
1
-
-
-
-
-
1
2
-
2
2
-
-
2
-
-
1
-
-
-
1
1
7
1
-
-
2
-
1
1
1
-
-
1
-
1
-
-
-
-
-
-
-
1
-
2
-
2
2
-
-
-
-
1
-
-
1
1
7
1
-
-
2
-
1
1
1
1
-
-
-
-
-
-
642345
Ballongue
-
Regulation of acetate kinase a ...
Clostridium acetobutylicum
FEMS Microbiol. Lett.
35
295-301
1986
-
-
-
-
-
-
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1
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1
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2
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1
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2
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286050
Yarlett
Butyrate formation from glucos ...
Dasytricha ruminantium
Biochem. J.
228
187-192
1985
-
-
-
-
-
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1
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1
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2
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1
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2
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1
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1
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2
-
-
-
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-
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-
-
-
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-
-
-
-
642348
Twarog
Role of butyryl phosphate in t ...
Clostridium butyricum, Clostridium pasteurianum, Clostridium tetanomorphum, Clostridium tyrobutyricum
J. Bacteriol.
86
112-117
1963
-
-
-
-
-
-
-
7
-
2
-
-
-
4
-
-
1
-
-
-
1
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
7
-
2
-
-
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-
-
1
-
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1
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
642343
Twarog
Enzymic phosphorylation of but ...
Clostridium butyricum
J. Biol. Chem.
237
2474-2477
1962
-
-
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2
4
-
5
-
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1
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-
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5
-
-
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-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
5
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-