BRENDA - Enzyme Database show
show all sequences of 2.7.2.7

Butyrate kinase from Clostridium acetobutylicum

Hartmanis, M.G.N.; J. Biol. Chem. 262, 617-621 (1987)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
DTT
presence of a reducing agent is required
Clostridium acetobutylicum
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetamide
20 mM; 49% inhibition
Clostridium acetobutylicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
14
-
Butanoate
pH 7.6
Clostridium acetobutylicum
14
-
valerate
pH 7.6
Clostridium acetobutylicum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
Km: 2.0 mM
Clostridium acetobutylicum
Mn2+
Km: 4.0 mM
Clostridium acetobutylicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
2 * 39000, SDS-PAGE
Clostridium acetobutylicum
85000
-
non-denaturing PAGE
Clostridium acetobutylicum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium acetobutylicum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Clostridium acetobutylicum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
402
-
-
Clostridium acetobutylicum
Storage Stability
Storage Stability
Organism
-20°C, 25 mM Tris-HCl, pH 7.6, 1 mM DTT, stable for several months
Clostridium acetobutylicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + acetate
6% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + acetyl phosphate
-
-
-
?
ATP + butanoate
-
642344
Clostridium acetobutylicum
ADP + butanoyl phosphate
-
-
-
r
ATP + isobutanoate
54% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + isobutanoyl phosphate
-
-
-
?
ATP + isovalerate
32% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + isopentanoyl phosphate
-
-
-
?
ATP + propionate
43% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + propanoyl phosphate
-
-
-
?
ATP + valerate
89% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + pentanoyl phosphate
-
-
-
?
ATP + vinyl acetate
23% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + vinylacetyl phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 39000, SDS-PAGE
Clostridium acetobutylicum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
20 min, 70% loss of activity in absence of 100 mM butanoate, complete protection against inactivation in presence of mM potassium butanoate
Clostridium acetobutylicum
60
-
1 min, complete loss of activity in absence of butanoate
Clostridium acetobutylicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
formation of butanoyl phosphate
Clostridium acetobutylicum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
pH 6: about 25% of maximal activity, pH 10.0: about 50% of maximal activity
Clostridium acetobutylicum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
9.5
very stable
Clostridium acetobutylicum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Clostridium acetobutylicum
isoelectric focusing
5.9
5.6
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
DTT
presence of a reducing agent is required
Clostridium acetobutylicum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetamide
20 mM; 49% inhibition
Clostridium acetobutylicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
14
-
Butanoate
pH 7.6
Clostridium acetobutylicum
14
-
valerate
pH 7.6
Clostridium acetobutylicum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
Km: 2.0 mM
Clostridium acetobutylicum
Mn2+
Km: 4.0 mM
Clostridium acetobutylicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
2 * 39000, SDS-PAGE
Clostridium acetobutylicum
85000
-
non-denaturing PAGE
Clostridium acetobutylicum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium acetobutylicum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
402
-
-
Clostridium acetobutylicum
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 25 mM Tris-HCl, pH 7.6, 1 mM DTT, stable for several months
Clostridium acetobutylicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + acetate
6% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + acetyl phosphate
-
-
-
?
ATP + butanoate
-
642344
Clostridium acetobutylicum
ADP + butanoyl phosphate
-
-
-
r
ATP + isobutanoate
54% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + isobutanoyl phosphate
-
-
-
?
ATP + isovalerate
32% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + isopentanoyl phosphate
-
-
-
?
ATP + propionate
43% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + propanoyl phosphate
-
-
-
?
ATP + valerate
89% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + pentanoyl phosphate
-
-
-
?
ATP + vinyl acetate
23% of the activity with butanoate
642344
Clostridium acetobutylicum
ADP + vinylacetyl phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 39000, SDS-PAGE
Clostridium acetobutylicum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
20 min, 70% loss of activity in absence of 100 mM butanoate, complete protection against inactivation in presence of mM potassium butanoate
Clostridium acetobutylicum
60
-
1 min, complete loss of activity in absence of butanoate
Clostridium acetobutylicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
formation of butanoyl phosphate
Clostridium acetobutylicum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
pH 6: about 25% of maximal activity, pH 10.0: about 50% of maximal activity
Clostridium acetobutylicum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
9.5
very stable
Clostridium acetobutylicum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Clostridium acetobutylicum
isoelectric focusing
5.9
5.6
Other publictions for EC 2.7.2.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703499
Sullivan
-
Structural correlations of act ...
Clostridium acetobutylicum
Enzyme Microb. Technol.
46
118-124
2010
-
-
-
-
14
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
14
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704285
Diao
Crystal structure of butyrate ...
Thermotoga maritima
J. Bacteriol.
191
2521-2529
2009
-
-
1
1
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706668
Diao
Open and closed conformations ...
Thermotoga maritima
Proteins
80
1712
2009
-
-
-
1
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691411
Senger
Genome-scale model for Clostri ...
Clostridium acetobutylicum
Biotechnol. Bioeng.
101
1036-1052
2008
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693994
Sillers
Metabolic engineering of the n ...
Clostridium acetobutylicum, Clostridium acetobutylicum M5
Metab. Eng.
10
321-332
2008
-
1
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677610
Paillard
Relation between phylogenetic ...
Butyrivibrio fibrisolvens, Butyrivibrio fibrisolvens ATCC 19171 / D1, Butyrivibrio hungatei, Butyrivibrio hungatei JK615T, Butyrivibrio proteoclasticus, Butyrivibrio proteoclasticus B316T
Antonie van Leeuwenhoek
91
417-422
2007
-
-
3
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681800
Tessmer
Heat-shock protein HspA mimics ...
Clostridium acetobutylicum
Microbiology
153
366-374
2007
-
2
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691537
Raz
Isolation and characterisation ...
Enterococcus durans
Br. J. Nutr.
97
725-734
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679539
Liu
-
Butyric acid and hydrogen prod ...
Clostridium tyrobutyricum
Enzyme Microb. Technol.
38
521-528
2006
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486475
Liu
Exploitation of butyrate kinas ...
Clostridium acetobutylicum
Appl. Microbiol. Biotechnol.
53
545-552
2000
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642349
Huang
Identification and characteriz ...
Clostridium acetobutylicum
J. Mol. Microbiol. Biotechnol.
2
33-38
2000
-
-
1
-
-
-
-
1
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
486471
Cary
Cloning and expression of Clos ...
Clostridium acetobutylicum
J. Bacteriol.
170
4613-4618
1988
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642344
Hartmanis
Butyrate kinase from Clostridi ...
Clostridium acetobutylicum
J. Biol. Chem.
262
617-621
1987
1
-
-
-
-
-
1
2
-
2
2
-
-
2
-
-
1
-
-
-
1
1
7
1
-
-
2
-
1
1
1
-
-
1
-
1
-
-
-
-
-
-
-
1
-
2
-
2
2
-
-
-
-
1
-
-
1
1
7
1
-
-
2
-
1
1
1
1
-
-
-
-
-
-
642345
Ballongue
-
Regulation of acetate kinase a ...
Clostridium acetobutylicum
FEMS Microbiol. Lett.
35
295-301
1986
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286050
Yarlett
Butyrate formation from glucos ...
Dasytricha ruminantium
Biochem. J.
228
187-192
1985
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642348
Twarog
Role of butyryl phosphate in t ...
Clostridium butyricum, Clostridium pasteurianum, Clostridium tetanomorphum, Clostridium tyrobutyricum
J. Bacteriol.
86
112-117
1963
-
-
-
-
-
-
-
7
-
2
-
-
-
4
-
-
1
-
-
-
1
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
-
-
1
-
-
1
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
642343
Twarog
Enzymic phosphorylation of but ...
Clostridium butyricum
J. Biol. Chem.
237
2474-2477
1962
-
-
-
-
-
-
2
4
-
5
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
5
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
1
-
-
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