Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.2.4 extracted from

  • Rastegari, H.; Chiani, M.; Akbarzadeh, A.; Cheraghi, S.; Saffari, Z.; Mehrabi, M.; Farhangi, A.; Ghassemi, S.
    Improvement in the production of L-lysine by overexpression of aspartokinase (ASK) in C. glutamicum ATCC 21799 (2013), Trop. J. Pharm. Res., 12, 51-56.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
aspartokinase gene (ask) by cloning into an Escherichia coli/Corynebacterium glutamicum shuttle expression vector. Recombinant vector is transformed into Escherichia coli DH5alpha and then into Corynebacterium glutamicum. The induction of recombinant vector by IPTG has an inhibitory effect on cell growth due to over-expression of the cloned gene. A 2fold increase in lysine production is observed by cloning of the ASK gene in Corynebacterium glutamicum rather than in Escherichia coli, due to the presence of lysine exporter channel which facilitates lysine extraction Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
SDS-PAGE, recombinant protein Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Synonyms

Synonyms Comment Organism
Ask
-
Corynebacterium glutamicum
aspartokinase
-
Corynebacterium glutamicum

General Information

General Information Comment Organism
malfunction A 2fold increase in lysine production is observed by cloning of the ASK gene in Corynebacterium glutamicum rather than in Escherichia coli, due to the presence of lysine exporter channel which facilitates lysine extraction Corynebacterium glutamicum