Cloned (Comment) | Organism |
---|---|
aspartokinase gene (ask) by cloning into an Escherichia coli/Corynebacterium glutamicum shuttle expression vector. Recombinant vector is transformed into Escherichia coli DH5alpha and then into Corynebacterium glutamicum. The induction of recombinant vector by IPTG has an inhibitory effect on cell growth due to over-expression of the cloned gene. A 2fold increase in lysine production is observed by cloning of the ASK gene in Corynebacterium glutamicum rather than in Escherichia coli, due to the presence of lysine exporter channel which facilitates lysine extraction | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
SDS-PAGE, recombinant protein | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
Ask | - |
Corynebacterium glutamicum |
aspartokinase | - |
Corynebacterium glutamicum |
General Information | Comment | Organism |
---|---|---|
malfunction | A 2fold increase in lysine production is observed by cloning of the ASK gene in Corynebacterium glutamicum rather than in Escherichia coli, due to the presence of lysine exporter channel which facilitates lysine extraction | Corynebacterium glutamicum |