Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Xenorhabdus bovienii |
Protein Variants | Comment | Organism |
---|---|---|
E257K | mutation of the conserved Glu-257 to Lys or the double mutations T359I/E257K render the enzyme insensitive to L-lysine with 86- and 112fold increases in IC50 values, respectively, when compared to the wild-type enzyme. E257K and E257K/T359I alleles exhibit a 1.2- to 1.7fold decrease in Vmax value and 2- to 6fold decrease in kcat/Km value for either substrate compared to wild-type | Xenorhabdus bovienii |
T359I | mutation increases the L-lysine IC50 value by 104fold, but no substantial differences are observed in kinetic parameters except lower Km ATP value compared to the wild-type enzyme. Seed-specific expression of the feedback-resistant mutant T359I or mutant E257K results in increases of free L-threonine levels of up to 100fold in R1 soybean seed when compared to wild-type | Xenorhabdus bovienii |
T359I/E257K | mutation of the conserved Glu-257 to Lys or the double mutations T359I/E257K render the enzyme insensitive to L-lysine with 86- and 112fold increases in IC50 values, respectively, when compared to the wild-type enzyme. E257K and E257K/T359I alleles exhibit a 1.2- to 1.7fold decrease in Vmax value and 2- to 6fold decrease in kcat/Km value for either substrate compared to wild-type | Xenorhabdus bovienii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | - |
Xenorhabdus bovienii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.62 | - |
ATP | pH 8.0, 30°C, mutant E257K/T359I | Xenorhabdus bovienii | |
1.82 | - |
ATP | pH 8.0, 30°C, mutant T359I | Xenorhabdus bovienii | |
1.89 | - |
ATP | pH 8.0, 30°C, mutant E257K | Xenorhabdus bovienii | |
2.31 | - |
L-aspartate | pH 8.0, 30°C, mutant T359I | Xenorhabdus bovienii | |
2.56 | - |
ATP | pH 8.0, 30°C, wild-type | Xenorhabdus bovienii | |
2.73 | - |
L-aspartate | pH 8.0, 30°C, wild-type | Xenorhabdus bovienii | |
3.06 | - |
L-aspartate | pH 8.0, 30°C, mutant E257K | Xenorhabdus bovienii | |
3.1 | - |
L-aspartate | pH 8.0, 30°C, mutant E257K/T359I | Xenorhabdus bovienii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenorhabdus bovienii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Xenorhabdus bovienii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Xenorhabdus bovienii | ADP + 4-phospho-L-aspartate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XbAK | - |
Xenorhabdus bovienii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Xenorhabdus bovienii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Xenorhabdus bovienii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.4 | - |
pH 8.0, 30°C, wild-type | Xenorhabdus bovienii | L-lysine | |
121.5 | - |
pH 8.0, 30°C, mutant E257K | Xenorhabdus bovienii | L-lysine | |
145.4 | - |
pH 8.0, 30°C, mutant T359I | Xenorhabdus bovienii | L-lysine | |
157.2 | - |
pH 8.0, 30°C, mutant E257K/T359I | Xenorhabdus bovienii | L-lysine |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44 | - |
L-aspartate | pH 8.0, 30°C, mutant E257K | Xenorhabdus bovienii | |
110 | - |
ATP | pH 8.0, 30°C, mutant E257K | Xenorhabdus bovienii | |
120 | - |
L-aspartate | pH 8.0, 30°C, mutant E257K/T359I | Xenorhabdus bovienii | |
170 | - |
ATP | pH 8.0, 30°C, mutant E257K/T359I | Xenorhabdus bovienii | |
270 | - |
L-aspartate | pH 8.0, 30°C, wild-type | Xenorhabdus bovienii | |
290 | - |
L-aspartate | pH 8.0, 30°C, mutant T359I | Xenorhabdus bovienii | |
310 | - |
ATP | pH 8.0, 30°C, wild-type | Xenorhabdus bovienii | |
370 | - |
ATP | pH 8.0, 30°C, mutant T359I | Xenorhabdus bovienii |