Literature summary for 2.7.2.4 extracted from

Rognes, S.E.; Dewaele, E.; Aas, S.F.; Jacobs, M.; Frankard, V.
Transcriptional and biochemical regulation of a novel Arabidopsis thaliana bifunctional aspartate kinase-homoserine dehydrogenase gene isolated by functional complementation of a yeast hom6 mutant (2003), Plant Mol. Biol., 51, 281-294.

Search for more literature for 2.7.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
by functional complementation of a Saccharomyces cerevisiae strain mutated in its homoserine dehydrogenase gene (hom6), expression in Escherichia coli, two of the three isolated clones are also able to complement a mutant yeast aspartate kinase gene (hom3), expression of the AK-HSDH gene in Arabidopsis thaliana (meristematic cells, leaves and stamens)	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
S2207A	phenotype Thr- Met- Ura-	Arabidopsis thaliana
S2207A/pM1-1	mutant S2207A complemented with plasmid pM1-1, phenotype Thr+ Met+ Ura+	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
L-alanine	no significant effect on AK activity at 5 and 20 mM	Arabidopsis thaliana
L-isoleucine	no significant effect on AK activity at 5 and 20 mM	Arabidopsis thaliana
L-lysine	no significant effect on AK activity at 5 and 20 mM	Arabidopsis thaliana
L-threonine	inhibition at 0.5-1.0 mM in complemented S2207A cells is variable, it does not exceed 67%	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-aspartate	Arabidopsis thaliana	-	ADP + phospho-L-aspartate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-
Arabidopsis thaliana	-	Saccharomyces cerevisiae M20-20D cells are transformed with A. thaliana cDNA, protein expressed and purified from Saccharomyces cerevisiae, all data refer to the recombinant yeast protein	-

Purification (Commentary)

Purification (Comment)	Organism
-	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-
meristem	-	Arabidopsis thaliana	-
stamen	-	Arabidopsis thaliana	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0013	-	mutant S2207A	Arabidopsis thaliana
0.0107	-	complemented mutant A2207A/pM1-1	Arabidopsis thaliana
0.0268	-	wild type X-2180-1A	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-aspartate	-	Arabidopsis thaliana	ADP + phospho-L-aspartate	-	?

Synonyms

Synonyms	Comment	Organism
AK	-	Arabidopsis thaliana
aspartate kinase	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)