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Literature summary for 2.7.2.3 extracted from

  • Barros-Alvarez, X.; Caceres, A.J.; Michels, P.A.; Concepcion, J.L.; Quinones, W.
    The phosphoglycerate kinase isoenzymes have distinct roles in the regulation of carbohydrate metabolism in Trypanosoma cruzi (2014), Exp. Parasitol., 143, 39-47.
    View publication on PubMed
Search for more literature for 2.7.2.3

Cloned(Commentary)

Cloned (Comment) Organism
gene pgk-A, quantitative RT-PCR enzyme expression analysis, recombinant expression of N-terminally His-tagged wild-type and truncated mutant enzymes and of the 80 amino acids long peptide corresponding to the PGKA N-terminal insertion in Escherichia coli strain BL21(DE3) Trypanosoma cruzi
gene pgk-B, quantitative RT-PCR enzyme expression analysis Trypanosoma cruzi
gene pgk-C, quantitative RT-PCR enzyme expression analysis Trypanosoma cruzi

Protein Variants

Protein Variants Comment Organism
additional information construction of a mutant not possessing the 80 amino-acids insertion in its N-terminal domain, the PGKA N-terminal 80 amino-acids insertion is deleted by site-directed mutagenesis, obtaining an internally truncated rPGKA with a molecular weight of 48 kDa Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
ATP isozyme PGKC is partly inhibited by 0.13 mM Trypanosoma cruzi
suramin
-
 Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Trypanosoma cruzi
0.01
-
 ATP isozyme PGKC, pH 7.5, 22°C Trypanosoma cruzi
0.174
-
 3-phospho-D-glycerate native isozyme PGKA, pH 7.5, 22°C Trypanosoma cruzi
0.192
-
 3-phospho-D-glycerate isozyme PGKC, pH 7.5, 22°C Trypanosoma cruzi
0.217
-
 ATP native isozyme PGKA, pH 7.5, 22°C Trypanosoma cruzi
0.236
-
 ATP recombinant wild-type isozyme PGKA, pH 7.5, 22°C Trypanosoma cruzi
0.63
-
 3-phospho-D-glycerate recombinant truncated isozyme PGKA mutant, pH 7.5, 22°C Trypanosoma cruzi
0.64
-
 ATP recombinant truncated isozyme PGKA mutant, pH 7.5, 22°C Trypanosoma cruzi
0.85
-
 3-phospho-D-glycerate recombinant wild-type isozyme PGKA, pH 7.5, 22°C Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Trypanosoma cruzi 5829
-
glycosome
-
 Trypanosoma cruzi 20015
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
-
 Trypanosoma cruzi
48000
-
 recombinant truncated mutant enzyme, gel filtration Trypanosoma cruzi
52000
-
-
 Trypanosoma cruzi
57000
-
 recombinant wild-type enzyme, gel filtration Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3-phospho-D-glycerate Trypanosoma cruzi
-
 ADP + 3-phospho-D-glyceroyl phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
 isozyme PGKC, gene pgk-C
-
Trypanosoma cruzi Q95PK8 isozyme PGKB, gene pgk-B
-
Trypanosoma cruzi Q95PK9 isozyme PGKA, gene pgk-A
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged wild-type and truncated mutant enzymes and 80 amino acids long peptide corresponding to the PGKA N-terminal insertion to homogeneity from Escherichia coli strain BL21(DE3) by metal affinity chromatography Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
-
 Trypanosoma cruzi
-
epimastigote
-
 Trypanosoma cruzi
-
additional information differential expression of the PGK isoenzymes in amastigote, trypomastigote and epimastigote forms, quantitative RT-PCR expression analysis, overview Trypanosoma cruzi
-
trypomastigote
-
 Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12
-
 purified truncated enzyme mutant, pH 7.5, 22°C Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
 Trypanosoma cruzi ADP + 3-phospho-D-glyceroyl phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 47000, wild-type isozyme PGKC, SDS-PAGE Trypanosoma cruzi
monomer 1 * 52000, wild-type isozyme PGKA, SDS-PAGE, 1 * 48000, truncated enzyme mutant, SDS-PAGE Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
PGK
-
 Trypanosoma cruzi
pgk-B
-
 Trypanosoma cruzi
PGKA
-
 Trypanosoma cruzi
PGKB
-
 Trypanosoma cruzi
PGKC
-
 Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Trypanosoma cruzi
8
-
 recombinant isozyme PGKA Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0101
-
 suramin versus ATP, pH 7.5, 22°C Trypanosoma cruzi
0.0121
-
 suramin versus 3-phospho-D-glycerate, pH 7.5, 22°C Trypanosoma cruzi
0.27
-
 ATP isozyme PGKC, pH 7.5, 22°C Trypanosoma cruzi