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Literature summary for 2.7.2.3 extracted from
- Correlation between conformational stability of the ternary enzyme-substrate complex and domain closure of 3-phosphoglycerate kinase (2005), FEBS J., 272, 1867-1885.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| W308F | mutant enzyme is fully active, thermal stability is slightly decreased with respect to wild-type enzyme | Saccharomyces cerevisiae |
| W308F/W333F | mutant enzyme is fully active, thermal stability is slightly decreased with respect to wild-type enzyme | Saccharomyces cerevisiae |
General Stability
| General Stability | Organism |
|---|---|
| enzyme shows highest thermal stability in ternary complex with 3-phosphoglycerate and MgADP-, due to domain closure | Sus scrofa |
| enzyme shows highest thermal stability in ternary complex with 3-phosphoglycerate and MgADP-, due to domain closure | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Sus scrofa | ADP + 3-phospho-D-glyceroyl phosphate | - |
? |  |
| ATP + 3-phospho-D-glycerate | - |
Saccharomyces cerevisiae | ADP + 3-phospho-D-glyceroyl phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-phosphoglycerate kinase | - |
Sus scrofa |
| 3-phosphoglycerate kinase | - |
Saccharomyces cerevisiae |
| PGK | - |
Sus scrofa |
| PGK | - |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
midpoint temperature Tm, without a ligand, carboxamidomethylated enzyme, value determined by differential scanning calometry | Sus scrofa |
| 48 | - |
midpoint temperature Tm, with MgATP2- as ligand, carboxamidomethylated enzyme, value determined by differential scanning calometry | Sus scrofa |
| 48 | - |
midpoint temperature Tm, without a ligand, mutant enzyme W308F/W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 49 | - |
midpoint temperature Tm, with MgADP- as ligand, carboxamidomethylated enzyme, value determined by differential scanning calometry | Sus scrofa |
| 49 | - |
midpoint temperature Tm, with MgATP2- as ligand, mutant enzyme W308F/W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 51 | - |
midpoint temperature Tm, with 3-phosphoglycerate and MgADP- as ligand, carboxamidomethylated enzyme | Sus scrofa |
| 51 | - |
midpoint temperature Tm, with 3-phosphoglycerate as ligand, mutant enzyme W308F/W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 52 | - |
midpoint temperature Tm, with 3-phosphoglycerate as ligand, carboxamidomethylated enzyme, value determined by differential scanning calometry | Sus scrofa |
| 52 | - |
midpoint temperature Tm, with MgADP- as ligand, mutant enzyme W308F/W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 53 | - |
midpoint temperature Tm, without a ligand, mutant enzyme W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 53 | - |
midpoint temperature Tm, without a ligand, unmodified enzyme, value determined by differential scanning calometry | Sus scrofa |
| 54 | - |
midpoint temperature Tm, mutant enzyme W308F/W333F, with 3-phosphoglycerate and MgADP- as ligand, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 55 | - |
midpoint temperature Tm, with MgATP2- as ligand, unmodified enzyme, value determined by differential scanning calometry | Sus scrofa |
| 56 | - |
midpoint temperature Tm, with MgATP2- as ligand, mutant enzyme W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 56 | - |
midpoint temperature Tm, without a ligand, wild-type enzyme, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 57 | - |
midpoint temperature Tm, with 3-phosphoglycerate as ligand, mutant enzyme W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 57 | - |
midpoint temperature Tm, with MgADP- as ligand, mutant enzyme W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 57 | - |
midpoint temperature Tm, with MgADP- as ligand, unmodified enzyme, value determined by differential scanning calometry | Sus scrofa |
| 58 | - |
midpoint temperature Tm, with 3-phosphoglycerate as ligand, unmodified enzyme, value determined by differential scanning calometry | Sus scrofa |
| 59 | - |
midpoint temperature Tm, mutant enzyme W333F, with 3-phosphoglycerate and MgADP- as ligand, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 59 | - |
midpoint temperature Tm, with 3-phosphoglycerate and MgADP- as ligand, unmodified enzyme, value determined by differential scanning calometry | Sus scrofa |
| 60 | - |
midpoint temperature Tm, with 3-phosphoglycerate as ligand, wild-type enzyme, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 60 | - |
midpoint temperature Tm, with MgATP2- as ligand, wild-type enzyme, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 61 | - |
midpoint temperature Tm, with MgADP- as ligand, wild-type enzymne, value determined by differential scanning calometry | Saccharomyces cerevisiae |
| 63 | - |
midpoint temperature Tm, with 3-phosphoglycerate and MgADP- as ligand, wild-type enzyme, value determined by differential scanning calometry | Saccharomyces cerevisiae |
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