Literature summary for 2.7.2.3 extracted from

Krishnan, P.; Fu, Q.; Lam, W.; Liou, J.Y.; Dutschman, G.; Cheng, Y.C.
Phosphorylation of pyrimidine deoxynucleoside analog diphosphates: selective phosphorylation of L-nucleoside analog diphosphates by 3-phosphoglycerate kinase (2002), J. Biol. Chem., 277, 5453-5459.

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Inhibitors

Inhibitors	Comment	Organism	Structure
1-beta-D-arabinofuranosylcytosine	-	Homo sapiens
1-beta-D-arabinofuranosylcytosine 5'-diphosphate	-	Homo sapiens
2',2'-difluorodeoxycytidine	-	Homo sapiens
2',2'-difluorodeoxycytidine 5'-diphosphate	i.e. gemcitabine	Homo sapiens
2',3'-dideoxy-2',3'-didehydro-beta-L(-)-5-fluorodeoxycytidine	-	Homo sapiens
2',3'-dideoxy-2',3'-didehydro-beta-L(-)-5-fluorodeoxycytidine 5'-diphosphate	-	Homo sapiens
2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil	-	Homo sapiens
2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil 5'-diphosphate	-	Homo sapiens
beta-L-2',3'-dideoxy-3'-thiacytidine	-	Homo sapiens
beta-L-2',3'-dideoxy-3'-thiacytidine diphosphate	-	Homo sapiens
D-2',3'-dideoxycytidine	-	Homo sapiens
L-2',3'-dideoxycytidine	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Saccharomyces cerevisiae	-	commercial product	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
Hep-G2 cell	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
184	-	purified enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2',3'-dideoxy-2',3'-didehydro-beta-L(-)-5-fluorodeoxycytidine 5'-triphosphate + 3-phospho-D-glycerate	isozyme PGK1	Saccharomyces cerevisiae	2',3'-dideoxy-2',3'-didehydro-beta-L(-)-5-fluorodeoxycytidine 5'-diphosphate + 1,3-diphosphoglycerate	-	?
2',3'-dideoxy-2',3'-didehydro-beta-L(-)-5-fluorodeoxycytidine 5'-triphosphate + 3-phospho-D-glycerate	3.6fold higher activity compared to 2'-deoxycytidine	Saccharomyces cerevisiae	2',3'-dideoxy-2',3'-didehydro-beta-L(-)-5-fluorodeoxycytidine 5'-diphosphate + 1,3-diphosphoglycerate	-	?
2'-deoxycytidine 5'-triphosphate + 3-phospho-D-glycerate	isozyme PGK1	Saccharomyces cerevisiae	2'-deoxycytidine 5'-diphosphate + 1,3-diphosphoglycerate	-	?
beta-L(-)-dioxolanecytidine 5'-triphosphate + 3-phospho-D-glycerate	isozyme PGK1	Saccharomyces cerevisiae	beta-L(-)-dioxolanecytidine 5'-diphosphate + 1,3-diphosphoglycerate	-	?
beta-L(-)-dioxolanecytidine 5'-triphosphate + 3-phospho-D-glycerate	3fold higher activity compared to 2'-deoxycytidine	Saccharomyces cerevisiae	beta-L(-)-dioxolanecytidine 5'-diphosphate + 1,3-diphosphoglycerate	-	?
beta-L-2',3'-dideoxy-3'-thiacytidine 5'-triphosphate + 3-phospho-D-glycerate	isozyme PGK1	Saccharomyces cerevisiae	beta-L-2',3'-dideoxy-3'-thiacytidine 5'-diphosphate + 1,3-diphosphoglycerate	-	?
beta-L-2',3'-dideoxy-3'-thiacytidine 5'-triphosphate + 3-phospho-D-glycerate	4fold higher activity compared to 2'-deoxycytidine	Saccharomyces cerevisiae	beta-L-2',3'-dideoxy-3'-thiacytidine 5'-diphosphate + 1,3-diphosphoglycerate	-	?
additional information	-	Homo sapiens	?	-	?
additional information	2',3'-dideoxycytidine is a very poor substrate	Saccharomyces cerevisiae	?	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.045	-	D-2',3'-dideoxycytidine	pH 7.5	Homo sapiens
0.065	-	2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil	pH 7.5	Homo sapiens
0.087	-	2',2'-difluorodeoxycytidine	pH 7.5	Homo sapiens
0.109	-	1-beta-D-arabinofuranosylcytosine	pH 7.5	Homo sapiens
0.12	-	2',3'-dideoxy-2',3'-didehydro-beta-L(-)-5-fluorodeoxycytidine	pH 7.5	Homo sapiens
0.125	-	L-2',3'-dideoxycytidine	pH 7.5	Homo sapiens
0.25	-	beta-L-2',3'-dideoxy-3'-thiacytidine	pH 7.5	Homo sapiens

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Release 2023.1 (January 2023)