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Literature summary for 2.7.2.3 extracted from

  • Bourniquel, A.A.; Mollet, B.
    Purification and characterization of the 3-phosphoglycerate kinase from the thermophile Lactobacillus delbrueckii subsp. lactis (2002), Int. Dairy J., 12, 723-728.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
ATP pH 6.8, 45°C Lactobacillus delbrueckii subsp. lactis
2.6
-
3-phospho-D-glycerate pH 6.8, 45°C Lactobacillus delbrueckii subsp. lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Lactobacillus delbrueckii subsp. lactis
46000
-
1 * 46000, SDS-PAGE Lactobacillus delbrueckii subsp. lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + 3-phospho-D-glyceroyl phosphate Lactobacillus delbrueckii subsp. lactis
-
ATP + 3-phospho-D-glycerate
-
r
ADP + 3-phospho-D-glyceroyl phosphate Lactobacillus delbrueckii subsp. lactis NCC88
-
ATP + 3-phospho-D-glycerate
-
r
additional information Lactobacillus delbrueckii subsp. lactis phylogenetic tree ?
-
?
additional information Lactobacillus delbrueckii subsp. lactis NCC88 phylogenetic tree ?
-
?

Organism

Organism UniProt Comment Textmining
Lactobacillus delbrueckii subsp. lactis
-
-
-
Lactobacillus delbrueckii subsp. lactis NCC88
-
-
-

Purification (Commentary)

Purification (Comment) Organism
77fold, to homogeneity Lactobacillus delbrueckii subsp. lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
605
-
purified enzyme Lactobacillus delbrueckii subsp. lactis

Storage Stability

Storage Stability Organism
4°C, purified enzyme, 20 mM Tris-HCl, pH 7.4, 50 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, at least 4 months stable Lactobacillus delbrueckii subsp. lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + 3-phospho-D-glyceroyl phosphate
-
Lactobacillus delbrueckii subsp. lactis ATP + 3-phospho-D-glycerate
-
r
ADP + 3-phospho-D-glyceroyl phosphate
-
Lactobacillus delbrueckii subsp. lactis NCC88 ATP + 3-phospho-D-glycerate
-
r
ATP + 3-phospho-D-glycerate
-
Lactobacillus delbrueckii subsp. lactis ADP + 1,3-diphosphoglycerate
-
r
ATP + 3-phospho-D-glycerate
-
Lactobacillus delbrueckii subsp. lactis NCC88 ADP + 1,3-diphosphoglycerate
-
r
additional information phylogenetic tree Lactobacillus delbrueckii subsp. lactis ?
-
?
additional information phylogenetic tree Lactobacillus delbrueckii subsp. lactis NCC88 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 46000, SDS-PAGE Lactobacillus delbrueckii subsp. lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Lactobacillus delbrueckii subsp. lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Lactobacillus delbrueckii subsp. lactis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.6 90% activity at pH 6.0 and pH 7.4, 70% activity at pH 5.5, about 35% activity at pH 7.6 Lactobacillus delbrueckii subsp. lactis

Cofactor

Cofactor Comment Organism Structure
ADP
-
Lactobacillus delbrueckii subsp. lactis
ATP required as phosphate donor Lactobacillus delbrueckii subsp. lactis