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Literature summary for 2.7.2.3 extracted from
- Isolation and characterization of the photosynthetic phosphoglycerate kinase from Beta vulgaris (1976), Eur. J. Biochem., 63, 483-490.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 77% of the activity with Mg2+ | Beta vulgaris |  |
| Zn2+ | - |
Beta vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | 0.2 | ATP | pH 7.5, 30°C | Beta vulgaris | |
| 0.15 | 0.2 | ATP | ATP in form of MgATP2- | Beta vulgaris | |
| 0.6 | 0.7 | 3-phospho-D-glycerate | pH 7.5, 30°C | Beta vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially replace Mg2+ in activation | Beta vulgaris | |
| Ca2+ | 34% of the activity with Mg2+ | Beta vulgaris | |
| Cd2+ | can partially replace Mg2+ in activation | Beta vulgaris | |
| Co2+ | can partially replace Mg2+ in activation | Beta vulgaris | |
| Co2+ | 77% of the activity with Mg2+ | Beta vulgaris | |
| Mg2+ | true substrate is the magnesium complexes of ATP | Beta vulgaris | |
| Mn2+ | can partially replace Mg2+ in activation | Beta vulgaris | |
| Mn2+ | 67% of the activity with Mg2+ | Beta vulgaris | |
| Ni2+ | can partially replace Mg2+ in activation | Beta vulgaris | |
| Ni2+ | 12% of the activity with Mg2+ | Beta vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
gel filtration | Beta vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Beta vulgaris | - |
silver beet | - |
| Beta vulgaris | - |
var. Fordhook giant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Beta vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Beta vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 900 | 980 | purified enzyme, depending on purification method | Beta vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Beta vulgaris | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Beta vulgaris | ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Beta vulgaris |
| monomer | 1 * 47000, SDS-PAGE | Beta vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Beta vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Beta vulgaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Beta vulgaris | |
| ATP | true substrate is the magnesium complexes of ATP | Beta vulgaris | |
| ATP | required as phosphate donor | Beta vulgaris | |
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