Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 77% of the activity with Mg2+ | Beta vulgaris | |
Zn2+ | - |
Beta vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | 0.2 | ATP | pH 7.5, 30°C | Beta vulgaris | |
0.15 | 0.2 | ATP | ATP in form of MgATP2- | Beta vulgaris | |
0.6 | 0.7 | 3-phospho-D-glycerate | pH 7.5, 30°C | Beta vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can partially replace Mg2+ in activation | Beta vulgaris | |
Ca2+ | 34% of the activity with Mg2+ | Beta vulgaris | |
Cd2+ | can partially replace Mg2+ in activation | Beta vulgaris | |
Co2+ | can partially replace Mg2+ in activation | Beta vulgaris | |
Co2+ | 77% of the activity with Mg2+ | Beta vulgaris | |
Mg2+ | true substrate is the magnesium complexes of ATP | Beta vulgaris | |
Mn2+ | can partially replace Mg2+ in activation | Beta vulgaris | |
Mn2+ | 67% of the activity with Mg2+ | Beta vulgaris | |
Ni2+ | can partially replace Mg2+ in activation | Beta vulgaris | |
Ni2+ | 12% of the activity with Mg2+ | Beta vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
gel filtration | Beta vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Beta vulgaris | - |
silver beet | - |
Beta vulgaris | - |
var. Fordhook giant | - |
Purification (Comment) | Organism |
---|---|
to homogeneity | Beta vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Beta vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
900 | 980 | purified enzyme, depending on purification method | Beta vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Beta vulgaris | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
Beta vulgaris | ADP + 3-phospho-D-glyceroyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | - |
Beta vulgaris |
monomer | 1 * 47000, SDS-PAGE | Beta vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Beta vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Beta vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Beta vulgaris | |
ATP | true substrate is the magnesium complexes of ATP | Beta vulgaris | |
ATP | required as phosphate donor | Beta vulgaris |