KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
ATP | pH 7.5, isozyme PGKA, in presence of 0.05 mM 1,3-bisphosphoglycerate | Ovis aries | |
0.09 | - |
ATP | ATP in form of MgATP2- | Ovis aries | |
0.095 | - |
ATP | pH 7.5, isozyme PGKB, in presence of 0.05 mM 1,3-bisphosphoglycerate | Ovis aries | |
0.095 | - |
ATP | ATP in form of MgATP2- | Ovis aries |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
isozyme PGKA and PGKB, tryptophan content | Ovis aries |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
ram | - |
Ovis aries | - |
testis-specific isozyme PGKB, isozyme PGKA | - |
Purification (Comment) | Organism |
---|---|
2 isozymes: PGKA and PGKB, from testis to homogeneity | Ovis aries |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | isozyme PGKA | Ovis aries | - |
testis | testis-specific isozyme PGKB, isozyme PGKA | Ovis aries | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
700 | - |
purified isozyme PGKA | Ovis aries |
720 | - |
purified isozyme PGKB | Ovis aries |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Ovis aries | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
Ovis aries | ADP + 3-phospho-D-glyceroyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Ovis aries |
monomer | 1 * 47000-47500, isozymes PGKA and PGKB, SDS-PAGE | Ovis aries |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal denaturation at pH 5.5 and pH 7.5 of the isoenzymes | Ovis aries |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Ovis aries |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Ovis aries | |
ATP | true substrate is the magnesium complexes of ATP | Ovis aries | |
ATP | required as phosphate donor | Ovis aries |