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Literature summary for 2.7.2.3 extracted from
- 3-Phosphoglycerate kinase isozymes and genetic variants from mouse (1982), Methods Enzymol., 90, 121-126.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics for 3-phospho-D-glycerate are biphasic | Mus musculus | |
| 0.028 | - |
3-phospho-D-glycerate | isozyme PGK2C | Mus musculus |  |
| 0.038 | - |
ATP | isoenzyme PGK2C | Mus musculus | |
| 0.039 | - |
ADP | isozyme PGK2C | Mus musculus | |
| 0.15 | - |
3-phospho-D-glycerate | isozyme PGK2B, ADP, isoenzyme PGK2A | Mus musculus | |
| 0.19 | - |
ADP | isozyme PGK2B | Mus musculus | |
| 0.2 | - |
3-phospho-D-glycerate | isoenzyme PGK2A | Mus musculus | |
| 0.22 | - |
ATP | isoenzyme PGK1 | Mus musculus | |
| 0.23 | - |
ADP | isozyme PGK1 | Mus musculus | |
| 0.37 | - |
ATP | isozyme PGK2B | Mus musculus | |
| 0.37 | - |
ATP | ATP in form of MgATP2- | Mus musculus | |
| 0.42 | - |
ATP | isozyme PGK2A | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
gel filtration | Mus musculus |
| 47000 | - |
isoenzymes PGK1, PGK2A, PGK2B, PGK2C | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
2 isozymes: X-chromosome encoded PGK1 and chromosome 17 encoded PGK2, 3 genetic variants of PGK2: PGK2A, PGK2B, PGK2C | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzyme PGK1 and all 3 genetic variants of PGK2 | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | isozyme PGK1 is found in all somatic tissues | Mus musculus | - |
| muscle | - |
Mus musculus | - |
| spermatozoon | isozyme PGK2 | Mus musculus | - |
| testis | mature | Mus musculus | - |
| testis | isozyme PGK2 | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
purified isozyme PGK2C | Mus musculus |
| 430 | - |
purified isozyme PGK2B | Mus musculus |
| 450 | - |
purified isozyme PGK2A | Mus musculus |
| 500 | - |
purified isozyme PGK1 | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Mus musculus | ADP + 1,3-diphosphoglycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Mus musculus | ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
| CTP + 3-phospho-D-glycerate | low activity | Mus musculus | CDP + 1,3-diphosphoglycerate | - |
? | |
| dATP + 3-phospho-D-glycerate | isozymes PGK2A, PGK2B, and PGK1 show low activity, isozyme PGK2C shows 119% of the activity with ATP | Mus musculus | dADP + 1,3-diphosphoglycerate | - |
? | |
| dGTP + 3-phospho-D-glycerate | isozyme PGK2C with lower activity, all other isozymes show slightly lower activity compared to ATP | Mus musculus | dGDP + 1,3-diphosphoglycerate | - |
? | |
| GTP + 3-phospho-D-glycerate | - |
Mus musculus | GDP + 1,3-diphosphoglycerate | - |
? | |
| UTP + 3-phospho-D-glycerate | - |
Mus musculus | UDP + 1,3-diphosphoglycerate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Mus musculus | |
| ATP | required as phosphate donor | Mus musculus | |
| CTP | - |
Mus musculus | |
| dATP | - |
Mus musculus | |
| dGTP | - |
Mus musculus | |
| GTP | - |
Mus musculus | |
| UTP | - |
Mus musculus | |
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