Literature summary for 2.7.2.2 extracted from

Manca de Nadra, M.C.; Pesce de Ruiz Holgado, A.A.; Oliver, G.
Carbamate kinase of Lactobacillus buchneri NCDO110. II. Kinetic studies and reaction mechanism (1987), Biotechnol. Appl. Biochem., 9, 141-145.

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Inhibitors

Inhibitors	Comment	Organism	Structure
MgADP-	competitive to ATP	Lentilactobacillus buchneri

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.57	-	ADP	30°C, pH 5.0	Lentilactobacillus buchneri
0.57	-	ADP	ADP in form of MgADP-	Lentilactobacillus buchneri
0.68	-	ADP	30°C, pH 5.0	Lentilactobacillus buchneri
0.68	-	ADP	ADP in form of MnADP-	Lentilactobacillus buchneri
1.1	-	Carbamoyl phosphate	cosubstrate MnADP-, 30°C, pH 5.0	Lentilactobacillus buchneri
1.43	-	Carbamoyl phosphate	cosubstrate MgADP-, 30°C, pH 5.0	Lentilactobacillus buchneri

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Lentilactobacillus buchneri
Mg2+	Mn2+ is as effective as Mg2+	Lentilactobacillus buchneri
Mn2+	can replace Mg2+	Lentilactobacillus buchneri
Mn2+	Mn2+ as effective as Mg2+	Lentilactobacillus buchneri

Organism

Organism	UniProt	Comment	Textmining
Lentilactobacillus buchneri	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O	random type reaction mechanism	Lentilactobacillus buchneri	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + carbamoyl phosphate	-	Lentilactobacillus buchneri	ATP + NH3 + CO2	-	r
ADP + carbamoyl phosphate	-	Lentilactobacillus buchneri	ATP + carbamate	-	?
ATP + NH3 + CO2	-	Lentilactobacillus buchneri	ADP + carbamoyl phosphate	-	r

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Release 2023.1 (January 2023)