Any feedback?
Literature summary for 2.7.2.2 extracted from
- Carbamate kinase of Lactobacillus buchneri NCDO110. I. Purification and properties (1986), Biotechnol. Appl. Biochem., 8, 46-52.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | - |
Lentilactobacillus buchneri |  |
| ATP | - |
Lentilactobacillus buchneri | |
| CTP | - |
Lentilactobacillus buchneri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.71 | - |
ADP | 30°C, pH 5.0 | Lentilactobacillus buchneri | |
| 0.71 | - |
ADP | ADP in form of MgADP- | Lentilactobacillus buchneri | |
| 1.17 | - |
ADP | 30°C, pH 5.0 | Lentilactobacillus buchneri | |
| 1.17 | - |
ADP | ADP in form of MnADP- | Lentilactobacillus buchneri | |
| 1.53 | - |
Carbamoyl phosphate | cosubstrate MnADP-, 30°C, pH 5.0 | Lentilactobacillus buchneri | |
| 1.63 | - |
Carbamoyl phosphate | cosubstrate MgADP-, 30°C, pH 5.0 | Lentilactobacillus buchneri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mn2+ or Mg2+ required, higher activity with Mn2+ | Lentilactobacillus buchneri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97000 | - |
gel electrophoresis | Lentilactobacillus buchneri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentilactobacillus buchneri | - |
NCDO110 | - |
| Lentilactobacillus buchneri NCDO110 | - |
NCDO110 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lentilactobacillus buchneri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 59.52 | - |
- |
Lentilactobacillus buchneri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 2 weeks, stable | Lentilactobacillus buchneri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + carbamoyl phosphate | - |
Lentilactobacillus buchneri | ATP + carbamate | - |
? | |
| ADP + carbamoyl phosphate | - |
Lentilactobacillus buchneri NCDO110 | ATP + carbamate | - |
? | |
| ATP + NH3 + CO2 | - |
Lentilactobacillus buchneri | ADP + carbamoyl phosphate | - |
? | |
| ATP + NH3 + CO2 | - |
Lentilactobacillus buchneri NCDO110 | ADP + carbamoyl phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Lentilactobacillus buchneri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 8 | 30°C, pH 5.0 | Lentilactobacillus buchneri |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.4 | - |
CTP | plus Mg2+, 30°C, pH 5.0 | Lentilactobacillus buchneri | |
| 6.2 | - |
ATP | plus Mn2+, 30°C, pH 5.0 | Lentilactobacillus buchneri | |
| 9.4 | - |
ATP | plus Mg2+, 30°C, pH 5.0 | Lentilactobacillus buchneri | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
