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Literature summary for 2.7.2.12 extracted from
- Acetate kinase (pyrophosphate). A fourth pyrophosphate-dependent kinase from Entamoeba histolytica (1975), Biochem. Biophys. Res. Commun., 66, 1389-1395.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
acetyl phosphate | pH 7, 30°C | Entamoeba histolytica |  |
| 2.2 | - |
phosphate | pH 7, 30°C | Entamoeba histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| MgCl2 | employed in assay mixture | Entamoeba histolytica | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
| Entamoeba histolytica H200 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Entamoeba histolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, under N2, stable for a week | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with ADP, GDP, UDP, IDP or CDP | Entamoeba histolytica | ? | - |
? | |
| additional information | no activity with ADP, GDP, UDP, IDP or CDP | Entamoeba histolytica H200 | ? | - |
? | |
| phosphate + acetyl phosphate | much greater activity in the direction of acetate formation | Entamoeba histolytica | diphosphate + acetate | - |
r | |
| phosphate + acetyl phosphate | much greater activity in the direction of acetate formation | Entamoeba histolytica H200 | diphosphate + acetate | - |
r | |
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