Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
I150T | the mutant is greatly insensitive to feedback inhibition, leading to L-proline overproduction. The relative activity of the variant is 59% in the presence of 100 mM L-proline. The specific activity of the variant is slightly reduced compared with the wild type enzyme | Saccharomyces cerevisiae |
Q79H | the mutation results in extreme desensitization to feedback inhibition by L-proline, leading to L-proline overproduction. The relative activity of the variant is 96% in the presence of 100 mM L-proline and 87% in the presence of 400 mM L-proline. The specific activity of the variant is slightly reduced compared with the wild type enzyme | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-proline | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM used in assay conditions | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate | Saccharomyces cerevisiae | - |
ADP + L-glutamate 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P32264 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
mutant enzyme Q79H, at pH 7.4 and 30°C | Saccharomyces cerevisiae |
14.3 | - |
mutant enzyme I150T, at pH 7.4 and 30°C | Saccharomyces cerevisiae |
14.3 | - |
wild type enzyme, at pH 7.4 and 30°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate | - |
Saccharomyces cerevisiae | ADP + L-glutamate 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamyl kinase | - |
Saccharomyces cerevisiae |
PRO1 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | key enzyme in proline biosynthesis | Saccharomyces cerevisiae |