Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.2.11 extracted from

  • Murakami, N.; Kotaka, A.; Isogai, S.; Ashida, K.; Nishimura, A.; Matsumura, K.; Hata, Y.; Ishida, H.; Takagi, H.
    Effects of a novel variant of the yeast gamma-glutamyl kinase Pro1 on its enzymatic activity and sake brewing (2020), J. Ind. Microbiol. Biotechnol., 47, 715-723 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
I150T the mutant is greatly insensitive to feedback inhibition, leading to L-proline overproduction. The relative activity of the variant is 59% in the presence of 100 mM L-proline. The specific activity of the variant is slightly reduced compared with the wild type enzyme Saccharomyces cerevisiae
Q79H the mutation results in extreme desensitization to feedback inhibition by L-proline, leading to L-proline overproduction. The relative activity of the variant is 96% in the presence of 100 mM L-proline and 87% in the presence of 400 mM L-proline. The specific activity of the variant is slightly reduced compared with the wild type enzyme Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
L-proline
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate Saccharomyces cerevisiae
-
ADP + L-glutamate 5-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P32264
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
mutant enzyme Q79H, at pH 7.4 and 30°C Saccharomyces cerevisiae
14.3
-
mutant enzyme I150T, at pH 7.4 and 30°C Saccharomyces cerevisiae
14.3
-
wild type enzyme, at pH 7.4 and 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate
-
Saccharomyces cerevisiae ADP + L-glutamate 5-phosphate
-
?

Synonyms

Synonyms Comment Organism
gamma-glutamyl kinase
-
Saccharomyces cerevisiae
PRO1
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism key enzyme in proline biosynthesis Saccharomyces cerevisiae