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Literature summary for 2.7.2.11 extracted from
- Glutamate kinase from Thermotoga maritima: characterization of a thermophilic enzyme for proline biosynthesis (2010), Extremophiles, 14, 409-415.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Thermotoga maritima |  |
| cis-4-hydroxy-L-proline | - |
Thermotoga maritima | |
| methyl L-proline | - |
Thermotoga maritima | |
| trans-4-hydroxy-L-proline | - |
Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
SDS-PAGE | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| (NH4)2SO4 precipitation and a single ion-exchange chromatography step are used | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate | - |
Thermotoga maritima | ADP + L-glutamate 5-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | gel filtration, 4 * 42000 | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutamate kinase | - |
Thermotoga maritima |
| proB | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 83 | - |
- |
Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 85 | the enzyme retains approximately 100, 90 and 65% of its activity when heated for 45 min at 70, 80 and 85°C, respectively | Thermotoga maritima |
| 90 | - |
at 90°C, tmGK displays around 40% of the activity when heated for 45 min | Thermotoga maritima |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | - |
Thermotoga maritima |
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Release 2023.1 (January 2023)
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