Protein Variants | Comment | Organism |
---|---|---|
A62T | drastic reduction in specific activity. 911fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
A62V | drastic reduction in specific activity. 188fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
D147G | reduction in catalytic activity. 2000fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
D162G | drastic reduction in specific activity. 611.1fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
D162N | drastic reduction in specific activity. 644fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
E153A | reduction in catalytic activity. 555.5fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
E153G | reduction in catalytic activity. 555.5fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
E153K | reduction in catalytic activity. 3444.4fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
I149F | drastic reduction in specific activity. 222.2fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
I149F | 222.2fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
I79T | reduction in catalytic activity. 21.1fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
L154S | reduction in catalytic activity. 1000fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
M94T | reduction in catalytic activity. 255.6fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
S159P | reduction in catalytic activity. 211.1fold increase in Ki-value for L-Pro compared to wild-type enzyme | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Pro | - |
Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate | Solanum lycopersicum | - |
ADP + L-glutamate 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate | - |
Solanum lycopersicum | ADP + L-glutamate 5-phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
L-Pro | wild-type enzyme | Solanum lycopersicum | |
1.9 | - |
L-Pro | mutant enzyme I79T | Solanum lycopersicum | |
17 | - |
L-Pro | mutant enzyme A62V | Solanum lycopersicum | |
19 | - |
L-Pro | mutant enzyme S159P | Solanum lycopersicum | |
20 | - |
L-Pro | mutant enzyme I149F | Solanum lycopersicum | |
23 | - |
L-Pro | mutant enzyme M94T | Solanum lycopersicum | |
50 | - |
L-Pro | mutant enzyme E153A or E153G | Solanum lycopersicum | |
55 | - |
L-Pro | mutant enzyme D162G | Solanum lycopersicum | |
58 | - |
L-Pro | mutant enzyme D162N | Solanum lycopersicum | |
82 | - |
L-Pro | mutant enzyme A62T | Solanum lycopersicum | |
90 | - |
L-Pro | mutant enzyme L154S | Solanum lycopersicum | |
180 | - |
L-Pro | mutant enzyme D147G | Solanum lycopersicum | |
310 | - |
L-Pro | mutant enzyme E153K | Solanum lycopersicum |