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Literature summary for 2.7.2.1 extracted from
- The EutQ and EutP proteins are novel acetate kinases involved in ethanolamine catabolism Physiological implications for the function of the ethanolamine metabolosome in Salmonella enterica (2016), Mol. Microbiol., 99, 497-511 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(lambdaDE3) cells | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
ATP | acetate kinase EutP, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 0.5 | - |
ATP | acetate kinase EutQ, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.7 | - |
acetate | acetate kinase EutP, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.7 | - |
acetate | acetate kinase EutQ, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Salmonella enterica subsp. enterica serovar Typhimurium | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + acetyl phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
ATP + acetate | - |
r | |
| ATP + acetate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
ADP + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1750 | - |
acetate kinase EutP, with GTP as cosubstrate, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium |
| 2400 | - |
acetate kinase EutP, with ATP as cosubstrate, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + acetyl phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ATP + acetate | - |
r | |
| ATP + acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | highest activity | Salmonella enterica subsp. enterica serovar Typhimurium | ADP + acetyl phosphate | - |
r | |
| GTP + acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | GDP + acetyl phosphate | - |
r | |
| additional information | no activity with AMP-PCP, CTP or TTP | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EutP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| EutQ | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 318 | - |
acetate | acetate kinase EutQ, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 434 | - |
ATP | acetate kinase EutQ, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 545 | - |
acetate | acetate kinase EutP, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 657 | - |
ATP | acetate kinase EutP, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | acetate kinase EutQ is required during anoxic growth of Salmonella enterica on ethanolamine and tetrathionate | Salmonella enterica subsp. enterica serovar Typhimurium |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 454 | - |
acetate | acetate kinase EutQ, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 778 | - |
acetate | acetate kinase EutP, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 868 | - |
ATP | acetate kinase EutQ, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 1314 | - |
ATP | acetate kinase EutP, at 40°C, pH not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
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