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Literature summary for 2.7.2.1 extracted from
- Acetate kinase-an enzyme of the postulated phosphoketolase pathway in Methylomicrobium alcaliphilum 20Z (2015), Antonie van Leeuwenhoek, 108, 965-974 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologous expression of the His6-tagged enzyme in Escherichia coli Rosetta (DE3) | Methylotuvimicrobium alcaliphilum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
acetyl phosphate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum |  |
| 5.6 | - |
acetate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | the activity is strongly dependent on Mg2+ (100%), Mn2+ (100%) or Co2+ (68 %) ions | Methylotuvimicrobium alcaliphilum | |
| Mg2+ | the activity is strongly dependent on Mg2+ (100%), Mn2+ (100%) or Co2+ (68 %) ions | Methylotuvimicrobium alcaliphilum | |
| Mn2+ | the activity is strongly dependent on Mg2+ (100%), Mn2+ (100%) or Co2+ (68 %) ions | Methylotuvimicrobium alcaliphilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43600 | - |
calculated from sequence | Methylotuvimicrobium alcaliphilum |
| 45000 | - |
recombinant His6-tagged enzyme, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
| 80000 | - |
native gradient electrophoresis | Methylotuvimicrobium alcaliphilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylotuvimicrobium alcaliphilum | G4T0C7 | - |
- |
| Methylotuvimicrobium alcaliphilum 20Z | G4T0C7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methylotuvimicrobium alcaliphilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + acetyl phosphate | 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation. The enzyme shows strong specificity to ATP and ADP | Methylotuvimicrobium alcaliphilum | ATP + acetate | - |
r | |
| ADP + acetyl phosphate | 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation. The enzyme shows strong specificity to ATP and ADP | Methylotuvimicrobium alcaliphilum 20Z | ATP + acetate | - |
r | |
| ATP + acetate | 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation. The enzyme shows strong specificity to ATP and ADP | Methylotuvimicrobium alcaliphilum | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation. The enzyme shows strong specificity to ATP and ADP | Methylotuvimicrobium alcaliphilum 20Z | ADP + acetyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 43600, calculated from sequence | Methylotuvimicrobium alcaliphilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACK | - |
Methylotuvimicrobium alcaliphilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Methylotuvimicrobium alcaliphilum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | enzyme activity is 5fold higher at 70°C compared to that at 30°C | Methylotuvimicrobium alcaliphilum |
| 55 | 75 | 55°C: about 50% of maximal activity, 75°C: about 85% of maximal activity | Methylotuvimicrobium alcaliphilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 50-70% activity is retained after a 3 h exposure at 30-70°C | Methylotuvimicrobium alcaliphilum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 233.3 | - |
acetate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
| 316.7 | - |
acetyl phosphate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Methylotuvimicrobium alcaliphilum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2500 | - |
acetate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
| 2879 | - |
acetyl phosphate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
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