Cloned (Comment) | Organism |
---|---|
gene ackA, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499 | Shewanella sp. |
gene ackA, recombinant His-tagged enzyme overexpression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, thermodynamics | Escherichia coli | |
additional information | - |
additional information | Michaelis-Menten kinetics, thermodynamics | Shewanella sp. | |
0.47 | - |
ADP | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
0.48 | - |
ATP | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
0.48 | - |
ATP | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
0.49 | - |
ATP | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
0.49 | - |
ADP | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
0.5 | - |
acetyl phosphate | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
0.5 | - |
ATP | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
0.5 | - |
ADP | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
0.5 | - |
ADP | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
0.53 | - |
acetyl phosphate | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
0.53 | - |
acetyl phosphate | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
0.53 | - |
acetyl phosphate | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
0.57 | - |
ATP | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
0.59 | - |
ATP | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
0.59 | - |
acetyl phosphate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
0.61 | - |
ADP | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
0.62 | - |
ADP | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
0.64 | - |
ATP | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
0.64 | - |
ADP | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
0.65 | - |
ATP | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
0.66 | - |
ADP | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
0.67 | - |
acetyl phosphate | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
0.69 | - |
acetyl phosphate | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
0.71 | - |
acetyl phosphate | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
1.61 | - |
acetate | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
1.63 | - |
acetate | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
1.71 | - |
acetate | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
1.77 | - |
acetate | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
29.3 | - |
acetate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
31.32 | - |
acetate | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
32.26 | - |
acetate | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
36.02 | - |
acetate | pH 7.5, 10°C, recombinant enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Shewanella sp. | |
Mg2+ | the enzyme requires Mn2+ or Mg2+ | Escherichia coli | |
Mn2+ | the enzyme requires Mn2+ or Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
- |
Escherichia coli |
43000 | - |
- |
Shewanella sp. |
43300 | - |
- |
Escherichia coli |
43700 | - |
- |
Shewanella sp. |
86000 | - |
gel filtration, recombinant enzyme | Escherichia coli |
86000 | - |
gel filtration, recombinant enzyme | Shewanella sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Escherichia coli | - |
ADP + acetyl phosphate | - |
r | |
ATP + acetate | Shewanella sp. | - |
ADP + acetyl phosphate | - |
r | |
ATP + acetate | Shewanella sp. AS-11 | - |
ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6A3 | gene ackA | - |
Shewanella sp. | E3W769 | gene ackA | - |
Shewanella sp. AS-11 | E3W769 | gene ackA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography | Escherichia coli |
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography | Shewanella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + hydroxylamine | - |
Escherichia coli | acetyl hydroxamate + phosphate | - |
? | |
acetyl phosphate + hydroxylamine | - |
Shewanella sp. | acetyl hydroxamate + phosphate | - |
? | |
acetyl phosphate + hydroxylamine | - |
Shewanella sp. AS-11 | acetyl hydroxamate + phosphate | - |
? | |
ATP + acetate | - |
Escherichia coli | ADP + acetyl phosphate | - |
r | |
ATP + acetate | - |
Shewanella sp. | ADP + acetyl phosphate | - |
r | |
ATP + acetate | the equilibrium lies far to the left side | Escherichia coli | ADP + acetyl phosphate | - |
r | |
ATP + acetate | - |
Shewanella sp. AS-11 | ADP + acetyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 42000, recombinant enzyme, SDS-PAGE, 2 * 43300, about, sequence calculation | Escherichia coli |
homodimer | 2 * 43000, recombinant enzyme, SDS-PAGE, 2 * 43700, about, sequence calculation | Shewanella sp. |
Synonyms | Comment | Organism |
---|---|---|
ackA | - |
Escherichia coli |
EAK | - |
Escherichia coli |
Sak | - |
Shewanella sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
about | Shewanella sp. |
70 | - |
about | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | purified recombinant His-tagged enzyme, pH 7.5, 50% of maximal activity remaining after 15 min at 55°C for the free enzyme, at 65°C for the acetate-bound enzyme, and at 70°C for the ATP-bound enzyme. Free enzyme is completely stable up to 40°C, inactivation of all enzymes at 80°C | Escherichia coli |
20 | 80 | purified recombinant His-tagged enzyme, pH 7.5, the enzyme is completely stable up to 30°C, half-life is about 40-50°C, inactivation at 80°C | Shewanella sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
195 | - |
acetate | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
205 | - |
ATP | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
235 | - |
ATP | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
245 | - |
acetate | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
300 | - |
acetate | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
305 | - |
ATP | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
350 | - |
ATP | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
385 | - |
acetate | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
560 | - |
acetate | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
565 | - |
ATP | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
745 | - |
ATP | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
760 | - |
acetate | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
875 | - |
acetyl phosphate | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
884 | - |
ADP | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
890 | - |
acetyl phosphate | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
892 | - |
ADP | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
1130 | - |
ATP | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
1165 | - |
acetate | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
1340 | - |
acetyl phosphate | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
1350 | - |
ADP | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
1420 | - |
acetate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
1500 | - |
ATP | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
1550 | - |
acetyl phosphate | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
1565 | - |
ADP | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
1700 | - |
ADP | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
1700 | - |
acetyl phosphate | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
2100 | - |
acetyl phosphate | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
2135 | - |
ADP | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
2150 | - |
acetyl phosphate | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
2178 | - |
ADP | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
3515 | - |
ADP | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
3600 | - |
acetyl phosphate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Shewanella sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Escherichia coli | |
ADP | - |
Shewanella sp. | |
ATP | - |
Escherichia coli | |
ATP | - |
Shewanella sp. |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is found in anaerobic as well as aerobic bacteria and Archaea. The enzyme belongs to the ASKHA phosphotransferase family (acetate and sugar kinases/Hsc70/actin) and is the best candidate for the common ancestor of this family | Shewanella sp. |
evolution | the enzyme is found in anaerobic as well as aerobic bacteria and domain of Archaea, and play a central role in major link in the carbon cycle. The enzyme is also participated in the production of major amount o ATP in most of the fermentative anaerobes. The enzyme belongs to the phosphotransferases of the ASKHA (acetate and sugar kinases/Hsc70/actin) and the best candidate for the common ancestor of this family | Escherichia coli |
metabolism | the enzyme plays a central role in major link in the carbon cycle. The enzyme also participates in the production of major amount of ATP in most of the fermentative anaerobes | Shewanella sp. |
additional information | salts bridges and cation-pi interactions in the enzyme, overview | Escherichia coli |
additional information | salts bridges and cation-pi interactions in the enzyme, overview | Shewanella sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.5 | - |
acetate | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
23.5 | - |
acetate | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
37.2 | - |
acetate | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
48.5 | - |
acetate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
127 | - |
acetate | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
144 | - |
acetate | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
178 | - |
acetate | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
198 | - |
acetate | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
390 | - |
ATP | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
510 | - |
ATP | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
625 | - |
ATP | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
785 | - |
ATP | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
869 | - |
ATP | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
1164 | - |
ATP | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
1290 | - |
acetyl phosphate | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
1351 | - |
ADP | pH 7.5, 10°C, recombinant enzyme | Escherichia coli | |
1650 | - |
acetyl phosphate | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
1768 | - |
ADP | pH 7.5, 10°C, recombinant enzyme | Shewanella sp. | |
1982 | - |
ATP | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
2183 | - |
acetyl phosphate | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
2445 | - |
ADP | pH 7.5, 15°C, recombinant enzyme | Escherichia coli | |
2528 | - |
acetyl phosphate | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
2542 | - |
ATP | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
2700 | - |
ADP | pH 7.5, 15°C, recombinant enzyme | Shewanella sp. | |
3207 | - |
acetyl phosphate | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
3209 | - |
acetyl phosphate | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
3470 | - |
ADP | pH 7.5, 20°C, recombinant enzyme | Shewanella sp. | |
3513 | - |
ADP | pH 7.5, 20°C, recombinant enzyme | Escherichia coli | |
4200 | - |
acetyl phosphate | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
4542 | - |
ADP | pH 7.5, 25°C, recombinant enzyme | Shewanella sp. | |
5762 | - |
ADP | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
6102 | - |
acetyl phosphate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli |