Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.2.1 extracted from

  • Tang, M.A.; Motoshima, H.; Watanabe, K.
    Cold adaptation: structural and functional characterizations of psychrophilic and mesophilic acetate kinase (2014), Protein J., 33, 313-322.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ackA, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499 Shewanella sp.
gene ackA, recombinant His-tagged enzyme overexpression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, thermodynamics Escherichia coli
additional information
-
additional information Michaelis-Menten kinetics, thermodynamics Shewanella sp.
0.47
-
ADP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
0.48
-
ATP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
0.48
-
ATP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
0.49
-
ATP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
0.49
-
ADP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
0.5
-
acetyl phosphate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
0.5
-
ATP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
0.5
-
ADP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
0.5
-
ADP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
0.53
-
acetyl phosphate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
0.53
-
acetyl phosphate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
0.53
-
acetyl phosphate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
0.57
-
ATP pH 7.5, 20°C, recombinant enzyme Escherichia coli
0.59
-
ATP pH 7.5, 25°C, recombinant enzyme Escherichia coli
0.59
-
acetyl phosphate pH 7.5, 25°C, recombinant enzyme Escherichia coli
0.61
-
ADP pH 7.5, 25°C, recombinant enzyme Escherichia coli
0.62
-
ADP pH 7.5, 20°C, recombinant enzyme Escherichia coli
0.64
-
ATP pH 7.5, 15°C, recombinant enzyme Escherichia coli
0.64
-
ADP pH 7.5, 15°C, recombinant enzyme Escherichia coli
0.65
-
ATP pH 7.5, 10°C, recombinant enzyme Escherichia coli
0.66
-
ADP pH 7.5, 10°C, recombinant enzyme Escherichia coli
0.67
-
acetyl phosphate pH 7.5, 20°C, recombinant enzyme Escherichia coli
0.69
-
acetyl phosphate pH 7.5, 10°C, recombinant enzyme Escherichia coli
0.71
-
acetyl phosphate pH 7.5, 15°C, recombinant enzyme Escherichia coli
1.61
-
acetate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
1.63
-
acetate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
1.71
-
acetate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
1.77
-
acetate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
29.3
-
acetate pH 7.5, 25°C, recombinant enzyme Escherichia coli
31.32
-
acetate pH 7.5, 20°C, recombinant enzyme Escherichia coli
32.26
-
acetate pH 7.5, 15°C, recombinant enzyme Escherichia coli
36.02
-
acetate pH 7.5, 10°C, recombinant enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Shewanella sp.
Mg2+ the enzyme requires Mn2+ or Mg2+ Escherichia coli
Mn2+ the enzyme requires Mn2+ or Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
-
Escherichia coli
43000
-
-
Shewanella sp.
43300
-
-
Escherichia coli
43700
-
-
Shewanella sp.
86000
-
gel filtration, recombinant enzyme Escherichia coli
86000
-
gel filtration, recombinant enzyme Shewanella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Escherichia coli
-
ADP + acetyl phosphate
-
r
ATP + acetate Shewanella sp.
-
ADP + acetyl phosphate
-
r
ATP + acetate Shewanella sp. AS-11
-
ADP + acetyl phosphate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6A3 gene ackA
-
Shewanella sp. E3W769 gene ackA
-
Shewanella sp. AS-11 E3W769 gene ackA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography Escherichia coli
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography Shewanella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl phosphate + hydroxylamine
-
Escherichia coli acetyl hydroxamate + phosphate
-
?
acetyl phosphate + hydroxylamine
-
Shewanella sp. acetyl hydroxamate + phosphate
-
?
acetyl phosphate + hydroxylamine
-
Shewanella sp. AS-11 acetyl hydroxamate + phosphate
-
?
ATP + acetate
-
Escherichia coli ADP + acetyl phosphate
-
r
ATP + acetate
-
Shewanella sp. ADP + acetyl phosphate
-
r
ATP + acetate the equilibrium lies far to the left side Escherichia coli ADP + acetyl phosphate
-
r
ATP + acetate
-
Shewanella sp. AS-11 ADP + acetyl phosphate
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 42000, recombinant enzyme, SDS-PAGE, 2 * 43300, about, sequence calculation Escherichia coli
homodimer 2 * 43000, recombinant enzyme, SDS-PAGE, 2 * 43700, about, sequence calculation Shewanella sp.

Synonyms

Synonyms Comment Organism
ackA
-
Escherichia coli
EAK
-
Escherichia coli
Sak
-
Shewanella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
about Shewanella sp.
70
-
about Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 80 purified recombinant His-tagged enzyme, pH 7.5, 50% of maximal activity remaining after 15 min at 55°C for the free enzyme, at 65°C for the acetate-bound enzyme, and at 70°C for the ATP-bound enzyme. Free enzyme is completely stable up to 40°C, inactivation of all enzymes at 80°C Escherichia coli
20 80 purified recombinant His-tagged enzyme, pH 7.5, the enzyme is completely stable up to 30°C, half-life is about 40-50°C, inactivation at 80°C Shewanella sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
195
-
acetate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
205
-
ATP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
235
-
ATP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
245
-
acetate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
300
-
acetate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
305
-
ATP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
350
-
ATP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
385
-
acetate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
560
-
acetate pH 7.5, 10°C, recombinant enzyme Escherichia coli
565
-
ATP pH 7.5, 10°C, recombinant enzyme Escherichia coli
745
-
ATP pH 7.5, 15°C, recombinant enzyme Escherichia coli
760
-
acetate pH 7.5, 15°C, recombinant enzyme Escherichia coli
875
-
acetyl phosphate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
884
-
ADP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
890
-
acetyl phosphate pH 7.5, 10°C, recombinant enzyme Escherichia coli
892
-
ADP pH 7.5, 10°C, recombinant enzyme Escherichia coli
1130
-
ATP pH 7.5, 20°C, recombinant enzyme Escherichia coli
1165
-
acetate pH 7.5, 20°C, recombinant enzyme Escherichia coli
1340
-
acetyl phosphate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
1350
-
ADP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
1420
-
acetate pH 7.5, 25°C, recombinant enzyme Escherichia coli
1500
-
ATP pH 7.5, 25°C, recombinant enzyme Escherichia coli
1550
-
acetyl phosphate pH 7.5, 15°C, recombinant enzyme Escherichia coli
1565
-
ADP pH 7.5, 15°C, recombinant enzyme Escherichia coli
1700
-
ADP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
1700
-
acetyl phosphate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
2100
-
acetyl phosphate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2135
-
ADP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
2150
-
acetyl phosphate pH 7.5, 20°C, recombinant enzyme Escherichia coli
2178
-
ADP pH 7.5, 20°C, recombinant enzyme Escherichia coli
3515
-
ADP pH 7.5, 25°C, recombinant enzyme Escherichia coli
3600
-
acetyl phosphate pH 7.5, 25°C, recombinant enzyme Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli
7.5
-
assay at Shewanella sp.

Cofactor

Cofactor Comment Organism Structure
ADP
-
Escherichia coli
ADP
-
Shewanella sp.
ATP
-
Escherichia coli
ATP
-
Shewanella sp.

General Information

General Information Comment Organism
evolution the enzyme is found in anaerobic as well as aerobic bacteria and Archaea. The enzyme belongs to the ASKHA phosphotransferase family (acetate and sugar kinases/Hsc70/actin) and is the best candidate for the common ancestor of this family Shewanella sp.
evolution the enzyme is found in anaerobic as well as aerobic bacteria and domain of Archaea, and play a central role in major link in the carbon cycle. The enzyme is also participated in the production of major amount o ATP in most of the fermentative anaerobes. The enzyme belongs to the phosphotransferases of the ASKHA (acetate and sugar kinases/Hsc70/actin) and the best candidate for the common ancestor of this family Escherichia coli
metabolism the enzyme plays a central role in major link in the carbon cycle. The enzyme also participates in the production of major amount of ATP in most of the fermentative anaerobes Shewanella sp.
additional information salts bridges and cation-pi interactions in the enzyme, overview Escherichia coli
additional information salts bridges and cation-pi interactions in the enzyme, overview Shewanella sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.5
-
acetate pH 7.5, 10°C, recombinant enzyme Escherichia coli
23.5
-
acetate pH 7.5, 15°C, recombinant enzyme Escherichia coli
37.2
-
acetate pH 7.5, 20°C, recombinant enzyme Escherichia coli
48.5
-
acetate pH 7.5, 25°C, recombinant enzyme Escherichia coli
127
-
acetate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
144
-
acetate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
178
-
acetate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
198
-
acetate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
390
-
ATP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
510
-
ATP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
625
-
ATP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
785
-
ATP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
869
-
ATP pH 7.5, 10°C, recombinant enzyme Escherichia coli
1164
-
ATP pH 7.5, 15°C, recombinant enzyme Escherichia coli
1290
-
acetyl phosphate pH 7.5, 10°C, recombinant enzyme Escherichia coli
1351
-
ADP pH 7.5, 10°C, recombinant enzyme Escherichia coli
1650
-
acetyl phosphate pH 7.5, 10°C, recombinant enzyme Shewanella sp.
1768
-
ADP pH 7.5, 10°C, recombinant enzyme Shewanella sp.
1982
-
ATP pH 7.5, 20°C, recombinant enzyme Escherichia coli
2183
-
acetyl phosphate pH 7.5, 15°C, recombinant enzyme Escherichia coli
2445
-
ADP pH 7.5, 15°C, recombinant enzyme Escherichia coli
2528
-
acetyl phosphate pH 7.5, 15°C, recombinant enzyme Shewanella sp.
2542
-
ATP pH 7.5, 25°C, recombinant enzyme Escherichia coli
2700
-
ADP pH 7.5, 15°C, recombinant enzyme Shewanella sp.
3207
-
acetyl phosphate pH 7.5, 20°C, recombinant enzyme Shewanella sp.
3209
-
acetyl phosphate pH 7.5, 20°C, recombinant enzyme Escherichia coli
3470
-
ADP pH 7.5, 20°C, recombinant enzyme Shewanella sp.
3513
-
ADP pH 7.5, 20°C, recombinant enzyme Escherichia coli
4200
-
acetyl phosphate pH 7.5, 25°C, recombinant enzyme Shewanella sp.
4542
-
ADP pH 7.5, 25°C, recombinant enzyme Shewanella sp.
5762
-
ADP pH 7.5, 25°C, recombinant enzyme Escherichia coli
6102
-
acetyl phosphate pH 7.5, 25°C, recombinant enzyme Escherichia coli