Cloned (Comment) | Organism |
---|---|
gene ack, DNA and amino acid sequence determination, RT-PCR analysis, transcriptional organization, phylogenetic analysis in Methylotrophic bacteria, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Methylotuvimicrobium alcaliphilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the dependence of the activity on acetate and acetyl phosphate concentrations obeys the Michaelis-Menten kinetics, whereas the dependence on ATP and ADP concentrations is sigmoidal | Methylotuvimicrobium alcaliphilum | |
0.11 | - |
acetyl phosphate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
1.28 | - |
ADP | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
2.36 | - |
ATP | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
5.6 | - |
acetate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partly substitute for Mn2+ or Mg2+ | Methylotuvimicrobium alcaliphilum | |
Mg2+ | dependence on Mg2+ or Mn2+ ions, Km values is 0.28 mM | Methylotuvimicrobium alcaliphilum | |
Mn2+ | dependence on Mg2+ or Mn2+ ions, Km values is 0.47 mM | Methylotuvimicrobium alcaliphilum | |
additional information | no activity with Ba2+, Ca2+, Zn2+, or Cu2+ | Methylotuvimicrobium alcaliphilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
- |
Methylotuvimicrobium alcaliphilum |
80000 | - |
recombinant His6-tagged enzyme, native PAGE | Methylotuvimicrobium alcaliphilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Methylotuvimicrobium alcaliphilum | - |
ADP + acetyl phosphate | - |
r | |
ATP + acetate | Methylotuvimicrobium alcaliphilum 20Z | - |
ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylotuvimicrobium alcaliphilum | G4T0C7 | gene ack | - |
Methylotuvimicrobium alcaliphilum 20Z | G4T0C7 | gene ack | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Methylotuvimicrobium alcaliphilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Methylotuvimicrobium alcaliphilum | ADP + acetyl phosphate | - |
r | |
ATP + acetate | the enzyme is 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation and shows strong specificity to ATP/ADP The enzyme has a remarkably high catalytic efficiency for acetate and ATP formation | Methylotuvimicrobium alcaliphilum | ADP + acetyl phosphate | - |
r | |
ATP + acetate | - |
Methylotuvimicrobium alcaliphilum 20Z | ADP + acetyl phosphate | - |
r | |
ATP + acetate | the enzyme is 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation and shows strong specificity to ATP/ADP The enzyme has a remarkably high catalytic efficiency for acetate and ATP formation | Methylotuvimicrobium alcaliphilum 20Z | ADP + acetyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, recombinant His6-tagged enzyme, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
Synonyms | Comment | Organism |
---|---|---|
ACK | - |
Methylotuvimicrobium alcaliphilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
recombinant enzyme | Methylotuvimicrobium alcaliphilum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 80 | activity range, profile overview | Methylotuvimicrobium alcaliphilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | purified recombinant enzyme, pH 8.0, 50-70% activity is retained after a 3 h exposure at 30-70°C | Methylotuvimicrobium alcaliphilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.28 | - |
ADP | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
2.36 | - |
ATP | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
233 | - |
acetate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum | |
3167 | - |
acetyl phosphate | pH 8.0, 30°C | Methylotuvimicrobium alcaliphilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | recombinant enzyme | Methylotuvimicrobium alcaliphilum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | activity range | Methylotuvimicrobium alcaliphilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | strong specificity to ATP/ADP | Methylotuvimicrobium alcaliphilum | |
ATP | strong specificity to ATP/ADP | Methylotuvimicrobium alcaliphilum | |
additional information | no activity with CTP, GTP, or UTP | Methylotuvimicrobium alcaliphilum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the postulated phosphoketolase pathway | Methylotuvimicrobium alcaliphilum |