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Literature summary for 2.7.2.1 extracted from

  • Halbedel, S.; Eilers, H.; Jonas, B.; Busse, J.; Hecker, M.; Engelmann, S.; Stuelke, J.
    Transcription in Mycoplasma pneumoniae: analysis of the promoters of the ackA and ldh genes (2007), J. Mol. Biol., 371, 596-607.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, reporter system used that allows cloning of promoter fragments in front of a promoterless lacZ gene and insertion in Mycoplasma pneumoniae Mycoplasma pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Mycoplasma pneumoniae
-
ADP + acetyl phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mycoplasma pneumoniae P75245 wild-type strain M129 and mutant strains
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Mycoplasma pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
transcription initiation and regulation controlled in a carbon source dependent manner, ackA gene encoding acetate kinase is strongly expressed in the presence of glucose, promoter mapping by primer extension, promoter recognition sites studied by promoter deletion analysis, -35 region seems to be of minor importance Mycoplasma pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
Mycoplasma pneumoniae ADP + acetyl phosphate
-
?
ATP + acetate transcriptional control of the ackA gene Mycoplasma pneumoniae ADP + acetyl phosphate
-
?