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Literature summary for 2.7.2.1 extracted from

  • Ingram-Smith, C.; Gorrell, A.; Lawrence, S.H.; Iyer, P.; Smith, K.; Ferry, J.G.
    Characterization of the acetate binding pocket in the Methanosarcina thermophila acetate kinase (2005), J. Bacteriol., 187, 2386-2394.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.1

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Methanosarcina thermophila

Protein Variants

Protein Variants Comment Organism
F179A reduced catalytic efficiency with all substrates tested Methanosarcina thermophila
L122A reduced catalytic efficiency with all substrates tested Methanosarcina thermophila
P232A reduced catalytic efficiency with all substrates tested Methanosarcina thermophila
V93A improved catalytic efficiency with propionate and butyrate Methanosarcina thermophila
V93G improved catalytic efficiency with propionate and butyrate Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0499
-
 ATP mutant V93A, pH 7.0 Methanosarcina thermophila
0.0506
-
 ATP mutant F179A, pH 7.0 Methanosarcina thermophila
0.0542
-
 ATP mutant P232A, pH 7.0 Methanosarcina thermophila
0.0576
-
 ATP mutant L122A, pH 7.0 Methanosarcina thermophila
0.0687
-
 ATP mutant V93G, pH 7.0 Methanosarcina thermophila
0.0713
-
 ATP wild-type, pH 7.0 Methanosarcina thermophila
0.15
-
 acetyl phosphate mutant F179A, pH 7.4 Methanosarcina thermophila
0.34
-
 acetyl phosphate wild-type, pH 7.4 Methanosarcina thermophila
0.5
-
 acetyl phosphate mutant L122A, pH 7.4 Methanosarcina thermophila
0.71
-
 acetyl phosphate mutant V93G, pH 7.4 Methanosarcina thermophila
0.74
-
 acetyl phosphate mutant V93A, pH 7.4 Methanosarcina thermophila
1.19
-
 acetyl phosphate mutant P232A, pH 7.4 Methanosarcina thermophila
1.5
-
 acetate wild-type, pH 7.0 Methanosarcina thermophila
6.2
-
 propionate mutant V93A, pH 7.0 Methanosarcina thermophila
10.4
-
 acetate mutant F179A, pH 7.0 Methanosarcina thermophila
10.7
-
 propionate mutant L122A, pH 7.0 Methanosarcina thermophila
11
-
 propionate mutant F179A, pH 7.0 Methanosarcina thermophila
14.4
-
 propionate wild-type, pH 7.0 Methanosarcina thermophila
19.5
-
 acetate mutant L122A, pH 7.0 Methanosarcina thermophila
21.4
-
 acetate mutant V93A, pH 7.0 Methanosarcina thermophila
25
-
 propionate mutant V93G, pH 7.0 Methanosarcina thermophila
32
-
 acetate mutant P232A, pH 7.0 Methanosarcina thermophila
33.4
-
 Butyrate mutant V93A, pH 7.0 Methanosarcina thermophila
39
-
 Butyrate wild-type, pH 7.0 Methanosarcina thermophila
39.5
-
 acetate mutant V93G, pH 7.0 Methanosarcina thermophila
46
-
 propionate mutant P232A, pH 7.0 Methanosarcina thermophila
63
-
 Butyrate mutant V93G, pH 7.0 Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + acetate = ADP + acetyl phosphate residue F179 is essential for catalysis Methanosarcina thermophila
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl phosphate + hydroxylamine
-
 Methanosarcina thermophila acetyl hydroxamate + phosphate
-
 r
ATP + acetate
-
 Methanosarcina thermophila ADP + acetyl phosphate
-
 r
ATP + butyrate
-
 Methanosarcina thermophila ADP + butyryl phosphate
-
 r
ATP + propionate
-
 Methanosarcina thermophila ADP + propionyl phosphate
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 Butyrate wild-type, pH 7.0 Methanosarcina thermophila
0.37
-
 propionate mutant F179A, pH 7.0 Methanosarcina thermophila
2.2
-
 ATP mutant F179A, pH 7.0 Methanosarcina thermophila
6.7
-
 propionate mutant L122A, pH 7.0 Methanosarcina thermophila
8.5
-
 propionate mutant P232A, pH 7.0 Methanosarcina thermophila
12
-
 acetyl phosphate mutant F179A, pH 7.4 Methanosarcina thermophila
42.4
-
 Butyrate mutant V93A, pH 7.0 Methanosarcina thermophila
132
-
 ATP mutant P232A, pH 7.0 Methanosarcina thermophila
150
-
 ATP mutant L122A, pH 7.0 Methanosarcina thermophila
218
-
 propionate wild-type, pH 7.0 Methanosarcina thermophila
294
-
 Butyrate mutant V93G, pH 7.0 Methanosarcina thermophila
347
-
 ATP mutant V93G, pH 7.0 Methanosarcina thermophila
840
-
 propionate mutant V93G, pH 7.0 Methanosarcina thermophila
844
-
 ATP mutant V93A, pH 7.0 Methanosarcina thermophila
1029
-
 propionate mutant V93A, pH 7.0 Methanosarcina thermophila
1039
-
 acetyl phosphate mutant L122A, pH 7.4 Methanosarcina thermophila
1055
-
 ATP wild-type, pH 7.0 Methanosarcina thermophila
2396
-
 acetyl phosphate mutant P232A, pH 7.4 Methanosarcina thermophila
2572
-
 acetyl phosphate mutant V93G, pH 7.4 Methanosarcina thermophila
2680
-
 acetyl phosphate wild-type, pH 7.4 Methanosarcina thermophila
3869
-
 acetyl phosphate mutant V93A, pH 7.4 Methanosarcina thermophila