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Literature summary for 2.7.2.1 extracted from
- Purification and characterization of homo- and hetero-dimeric acetate kinases from the sulfate-reducing bacterium Desulfovibrio vulgaris (2001), J. Biochem., 129, 411-421.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl phosphate | product inhibition | Desulfovibrio vulgaris |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.12 | - |
ADP | pH 7.5, 25°C | Desulfovibrio vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Desulfovibrio vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47800 | - |
1 * 49300 + 1 * 47800, isoenzyme AK-II, alphaSalphaL heterodimer, SDS-PAGE | Desulfovibrio vulgaris |
| 47800 | - |
2 * 47800, isoenzyme AK-I, alphaS2 homodimer, SDS-PAGE | Desulfovibrio vulgaris |
| 49300 | - |
1 * 49300 + 1 * 47800, isoenzyme AK-II, alphaSalphaL heterodimer, SDS-PAGE | Desulfovibrio vulgaris |
| 76400 | - |
isoenzyme AK-I, gel filtration | Desulfovibrio vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | Desulfovibrio vulgaris | - |
ADP + acetyl phosphate | - |
r | |
| ATP + acetate | Desulfovibrio vulgaris Miyazaki F. | - |
ADP + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfovibrio vulgaris | - |
Miyazaki F. | - |
| Desulfovibrio vulgaris Miyazaki F. | - |
Miyazaki F. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isoenzymes purified | Desulfovibrio vulgaris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.6 | - |
isoenzyme AK-II | Desulfovibrio vulgaris |
| 28.3 | - |
isoenzyme AK-I | Desulfovibrio vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, can be stored in 50 mM Tris-HCl, pH 7.4, 0.3 M NaCl and 2 mM dithiothreitol for several months without loss of activity | Desulfovibrio vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | - |
Desulfovibrio vulgaris | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | - |
Desulfovibrio vulgaris Miyazaki F. | ADP + acetyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 49300 + 1 * 47800, isoenzyme AK-II, alphaSalphaL heterodimer, SDS-PAGE | Desulfovibrio vulgaris |
| dimer | 2 * 47800, isoenzyme AK-I, alphaS2 homodimer, SDS-PAGE | Desulfovibrio vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | - |
Desulfovibrio vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | - |
Desulfovibrio vulgaris |
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