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Literature summary for 2.7.2.1 extracted from

  • Miles, R.D.; Iyer, P.P.; Ferry, J.G.
    Site-directed mutational analysis of active site residues in the acetate kinase from Methanosarcina thermophila (2001), J. Biol. Chem., 276, 45059-45064.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned and hyper-expressed in Escherichia coli Methanosarcina thermophila

Protein Variants

Protein Variants Comment Organism
D148A site-directed mutagenesis Methanosarcina thermophila
D148E site-directed mutagenesis Methanosarcina thermophila
D148N site-directed mutagenesis Methanosarcina thermophila
E384A site-directed mutagenesis Methanosarcina thermophila
K14A site-directed mutagenesis Methanosarcina thermophila
K14R site-directed mutagenesis Methanosarcina thermophila
N211A site-directed mutagenesis Methanosarcina thermophila
N7A site-directed mutagenesis Methanosarcina thermophila
S10A site-directed mutagenesis Methanosarcina thermophila
S11A site-directed mutagenesis Methanosarcina thermophila
S12A site-directed mutagenesis Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
ATP
-
Methanosarcina thermophila
2.13
-
ATP pH 7.4, 37°C, wild-type Methanosarcina thermophila
3
-
ATP pH 7.4, 37°C, mutant N211A Methanosarcina thermophila
3.1
-
ATP pH 7.4, 37°C, mutant D148A Methanosarcina thermophila
3.2
-
ATP pH 7.4, 37°C, mutant S10A Methanosarcina thermophila
3.62
-
ATP pH 7.4, 37°C, mutant K14R Methanosarcina thermophila
3.7
-
ATP pH 7.4, 37°C, mutant S12T Methanosarcina thermophila
4.3
-
ATP pH 7.4, 37°C, mutant S10T Methanosarcina thermophila
4.5
-
ATP pH 7.4, 37°C, mutant S11A Methanosarcina thermophila
5
-
ATP pH 7.4, 37°C, mutant N7D, D148E Methanosarcina thermophila
5.2
-
ATP pH 7.4, 37°C, mutant K14A Methanosarcina thermophila
5.8
-
ATP pH 7.4, 37°C, mutant S10T Methanosarcina thermophila
6.8
-
ATP pH 7.4, 37°C, mutant S11T Methanosarcina thermophila
7
-
ATP pH 7.4, 37°C, mutant N7A Methanosarcina thermophila
7.3
-
ATP pH 7.4, 37°C, mutant S12A Methanosarcina thermophila
7.7
-
ATP pH 7.4, 37°C, mutant E384A Methanosarcina thermophila
48
-
acetate pH 7.4, 37°C, wild-type Methanosarcina thermophila
66
-
acetate
-
Methanosarcina thermophila
111
-
acetate pH 7.4, 37°C, mutant N211A Methanosarcina thermophila
170
-
acetate pH 7.4, 37°C, mutant D148A Methanosarcina thermophila
200
-
acetate pH 7.4, 37°C, mutant S11A Methanosarcina thermophila
239
-
acetate pH 7.4, 37°C, mutant K14R Methanosarcina thermophila
266
-
acetate pH 7.4, 37°C, mutant S10T Methanosarcina thermophila
333
-
acetate pH 7.4, 37°C, mutant S12T Methanosarcina thermophila
390
-
acetate pH 7.4, 37°C, mutant D148N Methanosarcina thermophila
693
-
acetate pH 7.4, 37°C, mutant N7D Methanosarcina thermophila
814
-
acetate pH 7.4, 37°C, mutant S10A Methanosarcina thermophila
1573
-
acetate pH 7.4, 37°C, mutant S11T Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Methanosarcina thermophila
Mg2+
-
Methanosarcina thermophila
Mn2+
-
Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Methanosarcina thermophila
-
ADP + acetyl phosphate
-
r

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.28
-
mutant D148A Methanosarcina thermophila
3.4
-
mutant E384A Methanosarcina thermophila
9
-
mutant S10A Methanosarcina thermophila
13
-
mutant N7A Methanosarcina thermophila
19
-
mutant S12A Methanosarcina thermophila
102
-
mutant K14A Methanosarcina thermophila
324
-
mutant S11A Methanosarcina thermophila
539
-
mutant N211A Methanosarcina thermophila
584
-
wild-type Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
Methanosarcina thermophila ADP + acetyl phosphate
-
r