Cloned (Comment) | Organism |
---|---|
cloned and hyper-expressed in Escherichia coli | Methanosarcina thermophila |
Protein Variants | Comment | Organism |
---|---|---|
D148A | site-directed mutagenesis | Methanosarcina thermophila |
D148E | site-directed mutagenesis | Methanosarcina thermophila |
D148N | site-directed mutagenesis | Methanosarcina thermophila |
E384A | site-directed mutagenesis | Methanosarcina thermophila |
K14A | site-directed mutagenesis | Methanosarcina thermophila |
K14R | site-directed mutagenesis | Methanosarcina thermophila |
N211A | site-directed mutagenesis | Methanosarcina thermophila |
N7A | site-directed mutagenesis | Methanosarcina thermophila |
S10A | site-directed mutagenesis | Methanosarcina thermophila |
S11A | site-directed mutagenesis | Methanosarcina thermophila |
S12A | site-directed mutagenesis | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
ATP | - |
Methanosarcina thermophila | |
2.13 | - |
ATP | pH 7.4, 37°C, wild-type | Methanosarcina thermophila | |
3 | - |
ATP | pH 7.4, 37°C, mutant N211A | Methanosarcina thermophila | |
3.1 | - |
ATP | pH 7.4, 37°C, mutant D148A | Methanosarcina thermophila | |
3.2 | - |
ATP | pH 7.4, 37°C, mutant S10A | Methanosarcina thermophila | |
3.62 | - |
ATP | pH 7.4, 37°C, mutant K14R | Methanosarcina thermophila | |
3.7 | - |
ATP | pH 7.4, 37°C, mutant S12T | Methanosarcina thermophila | |
4.3 | - |
ATP | pH 7.4, 37°C, mutant S10T | Methanosarcina thermophila | |
4.5 | - |
ATP | pH 7.4, 37°C, mutant S11A | Methanosarcina thermophila | |
5 | - |
ATP | pH 7.4, 37°C, mutant N7D, D148E | Methanosarcina thermophila | |
5.2 | - |
ATP | pH 7.4, 37°C, mutant K14A | Methanosarcina thermophila | |
5.8 | - |
ATP | pH 7.4, 37°C, mutant S10T | Methanosarcina thermophila | |
6.8 | - |
ATP | pH 7.4, 37°C, mutant S11T | Methanosarcina thermophila | |
7 | - |
ATP | pH 7.4, 37°C, mutant N7A | Methanosarcina thermophila | |
7.3 | - |
ATP | pH 7.4, 37°C, mutant S12A | Methanosarcina thermophila | |
7.7 | - |
ATP | pH 7.4, 37°C, mutant E384A | Methanosarcina thermophila | |
48 | - |
acetate | pH 7.4, 37°C, wild-type | Methanosarcina thermophila | |
66 | - |
acetate | - |
Methanosarcina thermophila | |
111 | - |
acetate | pH 7.4, 37°C, mutant N211A | Methanosarcina thermophila | |
170 | - |
acetate | pH 7.4, 37°C, mutant D148A | Methanosarcina thermophila | |
200 | - |
acetate | pH 7.4, 37°C, mutant S11A | Methanosarcina thermophila | |
239 | - |
acetate | pH 7.4, 37°C, mutant K14R | Methanosarcina thermophila | |
266 | - |
acetate | pH 7.4, 37°C, mutant S10T | Methanosarcina thermophila | |
333 | - |
acetate | pH 7.4, 37°C, mutant S12T | Methanosarcina thermophila | |
390 | - |
acetate | pH 7.4, 37°C, mutant D148N | Methanosarcina thermophila | |
693 | - |
acetate | pH 7.4, 37°C, mutant N7D | Methanosarcina thermophila | |
814 | - |
acetate | pH 7.4, 37°C, mutant S10A | Methanosarcina thermophila | |
1573 | - |
acetate | pH 7.4, 37°C, mutant S11T | Methanosarcina thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Methanosarcina thermophila | |
Mg2+ | - |
Methanosarcina thermophila | |
Mn2+ | - |
Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Methanosarcina thermophila | - |
ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.28 | - |
mutant D148A | Methanosarcina thermophila |
3.4 | - |
mutant E384A | Methanosarcina thermophila |
9 | - |
mutant S10A | Methanosarcina thermophila |
13 | - |
mutant N7A | Methanosarcina thermophila |
19 | - |
mutant S12A | Methanosarcina thermophila |
102 | - |
mutant K14A | Methanosarcina thermophila |
324 | - |
mutant S11A | Methanosarcina thermophila |
539 | - |
mutant N211A | Methanosarcina thermophila |
584 | - |
wild-type | Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r |