Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.2.1 extracted from

  • Ingram-Smith, C.; Barber, R.D.; Ferry, J.G.
    The role of histidines in the acetate kinase from Methanosarcina thermophila (2000), J. Biol. Chem., 275, 33765-33770.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and variant acetate kinases are overproduced in Escherichia coli BL21(DE3) Methanosarcina thermophila

Protein Variants

Protein Variants Comment Organism
H123A site-directed mutagenesis Methanosarcina thermophila
H152A site-directed mutagenesis Methanosarcina thermophila
H180A site-directed mutagenesis Methanosarcina thermophila
H180R site-directed mutagenesis Methanosarcina thermophila
H184A site-directed mutagenesis Methanosarcina thermophila
H208A site-directed mutagenesis Methanosarcina thermophila
H60A site-directed mutagenesis Methanosarcina thermophila
H90A site-directed mutagenesis Methanosarcina thermophila
H94A site-directed mutagenesis Methanosarcina thermophila

Inhibitors

Inhibitors Comment Organism Structure
diethyldicarbonate
-
Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
ATP pH 7.0, 37°C, mutant H180A, produced in E. coli Methanosarcina thermophila
1.4
-
ATP pH 7.0, 37°C, mutant H180R, produced in E. coli Methanosarcina thermophila
1.5
-
ATP pH 7.0, 37°C, mutant H123A, produced in E. coli Methanosarcina thermophila
1.7
-
ATP pH 7.0, 37°C, mutant H90A, produced in E. coli Methanosarcina thermophila
1.9
-
ATP pH 7.0, 37°C, mutant H208A, produced in E. coli Methanosarcina thermophila
2
-
ATP pH 7.0, 37°C, unaltered wild-type enzyme, produced in E. coli Methanosarcina thermophila
2.7
-
ATP pH 7.0, 37°C, mutant H184A, produced in E. coli Methanosarcina thermophila
2.8
-
ATP pH 7.0, 37°C, authentic wild-type enzyme Methanosarcina thermophila
3
-
ATP pH 7.0, 37°C, mutant H60A, produced in E. coli Methanosarcina thermophila
3.7
-
ATP pH 7.0, 37°C, mutant H180Q, produced in E. coli Methanosarcina thermophila
4.6
-
ATP pH 7.0, 37°C, mutant H180N, produced in E. coli Methanosarcina thermophila
4.7
-
ATP pH 7.0, 37°C, mutant H180D, produced in E. coli Methanosarcina thermophila
5.8
-
ATP pH 7.0, 37°C, mutant H94A, produced in E. coli Methanosarcina thermophila
6.2
-
ATP pH 7.0, 37°C, mutant H180E, produced in E. coli Methanosarcina thermophila
6.3
-
ATP pH 7.0, 37°C, mutant H180K, produced in E. coli Methanosarcina thermophila
10
-
acetate pH 7.0, 37°C, mutant H180R, produced in E. coli Methanosarcina thermophila
13
-
acetate pH 7.0, 37°C, mutant H180D, produced in E. coli Methanosarcina thermophila
14
-
acetate pH 7.0, 37°C, mutant H180Q, produced in E. coli Methanosarcina thermophila
15
-
acetate pH 7.0, 37°C, mutant H184A, produced in E. coli Methanosarcina thermophila
16
-
acetate pH 7.0, 37°C, mutant H123A, produced in E. coli Methanosarcina thermophila
17
-
acetate pH 7.0, 37°C, mutant H180E, H180N, produced in E. coli Methanosarcina thermophila
18
-
acetate pH 7.0, 37°C, unaltered wild-type enzyme, produced in E. coli Methanosarcina thermophila
19
-
acetate pH 7.0, 37°C, mutant H180K, produced in E. coli Methanosarcina thermophila
20
-
acetate pH 7.0, 37°C, mutant H60A, produced in E. coli Methanosarcina thermophila
22
-
acetate pH 7.0, 37°C, authentic wild-type enzyme Methanosarcina thermophila
33
-
acetate pH 7.0, 37°C, mutant H208A, produced in E. coli Methanosarcina thermophila
56
-
ATP pH 7.0, 37°C, mutant H180A, produced in E. coli Methanosarcina thermophila
66
-
acetate pH 7.0, 37°C, mutant H94A, produced in E. coli Methanosarcina thermophila
330
-
acetate pH 7.0, 37°C, mutant H90A, produced in E. coli Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina thermophila