Cloned (Comment) | Organism |
---|---|
wild-type and mutant acetate kinase genes subcloned in to the T7-based expression vector pET15b | Methanosarcina thermophila |
Protein Variants | Comment | Organism |
---|---|---|
E384A | site-directed mutagenesis | Methanosarcina thermophila |
E384D | site-directed mutagenesis | Methanosarcina thermophila |
E384Q | site-directed mutagenesis | Methanosarcina thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inactivated by N-ethylmaleimide | Methanosarcina thermophila | |
N-ethylmaleimide | - |
Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ATP | pH 7.0, 20°C, mutant E334A, produced in E. coli | Methanosarcina thermophila | |
1 | - |
ATP | pH 7.0, 20°C, unaltered enzyme, produced in E. coli | Methanosarcina thermophila | |
1.1 | - |
ATP | pH 7.0, 20°C, mutant E32A, produced in E. coli | Methanosarcina thermophila | |
1.4 | - |
ATP | pH 7.0, 20°C, mutants E97A, E97D, E97Q, produced in E. coli | Methanosarcina thermophila | |
1.6 | - |
ATP | pH 7.0, 20°C, mutant E385C, produced in E. coli | Methanosarcina thermophila | |
2 | - |
ATP | pH 7.0, 20°C, mutant E385Q, produced in E. coli | Methanosarcina thermophila | |
2.3 | - |
acetate | pH 7.0, 20°C, mutant E97A, produced in E. coli | Methanosarcina thermophila | |
2.8 | - |
ATP | - |
Methanosarcina thermophila | |
4 | - |
acetate | pH 7.0, 20°C, mutant E97Q, produced in E. coli | Methanosarcina thermophila | |
4.3 | - |
ATP | pH 7.0, 20°C, mutant E385D, produced in E. coli | Methanosarcina thermophila | |
4.5 | - |
ATP | - |
Methanosarcina thermophila | |
6.9 | - |
ATP | pH 7.0, 20°C, mutant E385A, produced in E. coli | Methanosarcina thermophila | |
7.2 | - |
acetate | pH 7.0, 20°C, mutant E97D, produced in E. coli | Methanosarcina thermophila | |
15 | - |
acetate | pH 7.0, 20°C, mutant E334A, produced in E. coli | Methanosarcina thermophila | |
17 | - |
acetate | pH 7.0, 20°C, mutant E385C, produced in E. coli | Methanosarcina thermophila | |
19 | - |
acetate | pH 7.0, 20°C, unaltered enzyme, produced in E. coli | Methanosarcina thermophila | |
22 | - |
acetate | - |
Methanosarcina thermophila | |
26 | - |
acetate | pH 7.0, 20°C, mutant E32A, produced in E. coli | Methanosarcina thermophila | |
44 | - |
acetate | pH 7.0, 20°C, mutant E385Q, produced in E. coli | Methanosarcina thermophila | |
86 | - |
acetate | pH 7.0, 20°C, mutant E385A, produced in E. coli | Methanosarcina thermophila | |
337 | - |
acetate | pH 7.0, 20°C, mutant E385D, produced in E. coli | Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Methanosarcina thermophila | - |
ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
mutant E384A | Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Methanosarcina thermophila |