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Literature summary for 2.7.2.1 extracted from

  • Singh-Wissmann, K.; Ingram-Smith, C.; Miles, R.D.; Ferry, J.G.
    Identification of essential glutamates in the acetate kinase from Methanosarcina thermophila (1998), J. Bacteriol., 180, 1129-1134.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.1

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant acetate kinase genes subcloned in to the T7-based expression vector pET15b Methanosarcina thermophila

Protein Variants

Protein Variants Comment Organism
E384A site-directed mutagenesis Methanosarcina thermophila
E384D site-directed mutagenesis Methanosarcina thermophila
E384Q site-directed mutagenesis Methanosarcina thermophila

Inhibitors

Inhibitors Comment Organism Structure
additional information not inactivated by N-ethylmaleimide Methanosarcina thermophila
N-ethylmaleimide
-
 Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 ATP pH 7.0, 20°C, mutant E334A, produced in E. coli Methanosarcina thermophila
1
-
 ATP pH 7.0, 20°C, unaltered enzyme, produced in E. coli Methanosarcina thermophila
1.1
-
 ATP pH 7.0, 20°C, mutant E32A, produced in E. coli Methanosarcina thermophila
1.4
-
 ATP pH 7.0, 20°C, mutants E97A, E97D, E97Q, produced in E. coli Methanosarcina thermophila
1.6
-
 ATP pH 7.0, 20°C, mutant E385C, produced in E. coli Methanosarcina thermophila
2
-
 ATP pH 7.0, 20°C, mutant E385Q, produced in E. coli Methanosarcina thermophila
2.3
-
 acetate pH 7.0, 20°C, mutant E97A, produced in E. coli Methanosarcina thermophila
2.8
-
 ATP
-
 Methanosarcina thermophila
4
-
 acetate pH 7.0, 20°C, mutant E97Q, produced in E. coli Methanosarcina thermophila
4.3
-
 ATP pH 7.0, 20°C, mutant E385D, produced in E. coli Methanosarcina thermophila
4.5
-
 ATP
-
 Methanosarcina thermophila
6.9
-
 ATP pH 7.0, 20°C, mutant E385A, produced in E. coli Methanosarcina thermophila
7.2
-
 acetate pH 7.0, 20°C, mutant E97D, produced in E. coli Methanosarcina thermophila
15
-
 acetate pH 7.0, 20°C, mutant E334A, produced in E. coli Methanosarcina thermophila
17
-
 acetate pH 7.0, 20°C, mutant E385C, produced in E. coli Methanosarcina thermophila
19
-
 acetate pH 7.0, 20°C, unaltered enzyme, produced in E. coli Methanosarcina thermophila
22
-
 acetate
-
 Methanosarcina thermophila
26
-
 acetate pH 7.0, 20°C, mutant E32A, produced in E. coli Methanosarcina thermophila
44
-
 acetate pH 7.0, 20°C, mutant E385Q, produced in E. coli Methanosarcina thermophila
86
-
 acetate pH 7.0, 20°C, mutant E385A, produced in E. coli Methanosarcina thermophila
337
-
 acetate pH 7.0, 20°C, mutant E385D, produced in E. coli Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Methanosarcina thermophila
-
 ADP + acetyl phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
 mutant E384A Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
 Methanosarcina thermophila ADP + acetyl phosphate
-
 r

Subunits

Subunits Comment Organism
dimer
-
 Methanosarcina thermophila