Application | Comment | Organism |
---|---|---|
industry | enzyme involved in the wasteful production of acetate during conversion of cellulose to ethanol | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
135 | - |
acetate | pH 7.2-8.0, 50°C | Acetivibrio thermocellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Acetivibrio thermocellus | |
Mn2+ | - |
Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000, homodimer, SDS-PAGE | Acetivibrio thermocellus |
84000 | - |
gel filtration | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Acetivibrio thermocellus | - |
ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
ATCC 27405 | - |
Purification (Comment) | Organism |
---|---|
- |
Acetivibrio thermocellus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
490 | - |
- |
Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Acetivibrio thermocellus | ADP + acetyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46000, homodimer, SDS-PAGE | Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Acetivibrio thermocellus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | retains full activity, stable up to 60°C, loses 55% of its activity at 70°C, completely inactivated after 2 h at 80°C | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8 | - |
Acetivibrio thermocellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Acetivibrio thermocellus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | quite stable between pH 7.0 to 9.0, only 15% loss in activity at pH 10.0, inactivated rapidly outside this range | Acetivibrio thermocellus |