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Literature summary for 2.7.2.1 extracted from
- Acetate kinase from Clostridium acetobutylicum: a highly specific enzyme that is actively transcribed during acidogenesis and solventogenesis (1997), Microbiology, 143, 3279-3286.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA fragment encoding 246 bp of the acetate kinase gene of strain DSM 792 cloned by a PCR-based approach | Clostridium acetobutylicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | - |
Clostridium acetobutylicum |  |
| p-hydroxymercuribenzoate | - |
Clostridium acetobutylicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.37 | - |
ATP | pH 7.3, 30°C | Clostridium acetobutylicum | |
| 0.58 | - |
acetyl phosphate | pH 7.3, 30°C | Clostridium acetobutylicum | |
| 0.71 | - |
ADP | pH 7.3, 30°C | Clostridium acetobutylicum | |
| 73 | - |
acetate | pH 7.3, 30°C | Clostridium acetobutylicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Clostridium acetobutylicum | |
| Co2+ | - |
Clostridium acetobutylicum | |
| Mn2+ | - |
Clostridium acetobutylicum | |
| additional information | no activity with Zn2+ | Clostridium acetobutylicum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
2 * 43000, SDS-PAGE | Clostridium acetobutylicum |
| 87000 | 94000 | gel filtration, native gel electrophoresis | Clostridium acetobutylicum |
| 89000 | - |
native PAGE | Clostridium acetobutylicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | Clostridium acetobutylicum | - |
ADP + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | - |
DSM 1731 and DSM 792 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium acetobutylicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1087 | - |
- |
Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | - |
Clostridium acetobutylicum | ADP + acetyl phosphate | - |
r | |
| ATP + propionate | - |
Clostridium acetobutylicum | ADP + propionyl phosphate | - |
r | |
| CTP + acetate | - |
Clostridium acetobutylicum | CDP + acetyl phosphate | - |
r | |
| GTP + acetate | - |
Clostridium acetobutylicum | GDP + acetyl phosphate | - |
r | |
| ITP + acetate | - |
Clostridium acetobutylicum | IDP + acetyl phosphate | - |
r | |
| additional information | formate and butyrate are no substrates | Clostridium acetobutylicum | ? | - |
? | |
| UTP + acetate | - |
Clostridium acetobutylicum | UDP + acetyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000, SDS-PAGE | Clostridium acetobutylicum |
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