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Literature summary for 2.7.2.1 extracted from
- The acetate kinase of Clostridium acetobutylicum strain P262 (1996), Arch. Microbiol., 166, 418-420.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | iodoacetate and iodoacetamide does not inhibit | Clostridium acetobutylicum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
ATP | pH 7.0, 36°C | Clostridium acetobutylicum |  |
| 6 | - |
ADP | pH 7.0, 36°C | Clostridium acetobutylicum | |
| 160 | - |
acetate | pH 7.0, 36°C | Clostridium acetobutylicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strict requirement | Clostridium acetobutylicum | |
| Mn2+ | - |
Clostridium acetobutylicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE | Clostridium acetobutylicum |
| 78000 | - |
gel filtration | Clostridium acetobutylicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | - |
P262 | - |
| Clostridium acetobutylicum P262 | - |
P262 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium acetobutylicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27.7 | - |
- |
Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + propionate | 40% lower kinase activity than acetate | Clostridium acetobutylicum | ADP + propionyl phosphate | - |
r | |
| ATP + propionate | 40% lower kinase activity than acetate | Clostridium acetobutylicum P262 | ADP + propionyl phosphate | - |
r | |
| additional information | butyrate is no substrate | Clostridium acetobutylicum | ? | - |
? | |
| additional information | butyrate is no substrate | Clostridium acetobutylicum P262 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, SDS-PAGE | Clostridium acetobutylicum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 238 | - |
ATP | pH 7.0, 36°C | Clostridium acetobutylicum | |
| 280 | - |
acetate | pH 7.0, 36°C | Clostridium acetobutylicum | |
| 532 | - |
ADP | pH 7.0, 36°C | Clostridium acetobutylicum | |
| 700 | - |
acetyl phosphate | pH 7.0, 36°C | Clostridium acetobutylicum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
30% activity still observed at pH 5.5 | Clostridium acetobutylicum |
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